A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes

YajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR...

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Main Authors: Han, Sen, Shen, Danyu, Wang, Yu-Chuan, Chou, Shan-Ho, Gomelsky, Mark, Gao, Yong-Gui, Qian, Guoliang
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/142259
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1422592020-06-18T02:46:45Z A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes Han, Sen Shen, Danyu Wang, Yu-Chuan Chou, Shan-Ho Gomelsky, Mark Gao, Yong-Gui Qian, Guoliang School of Biological Sciences Science::Biological sciences Antifungal Antibiotic CdgL YajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a YajQ-LysR-like system in the bacterial biocontrol agent Lysobacter enzymogenes OH11 that secretes an antifungal antibiotic, heat-stable antifungal factor (HSAF) against crop fungal pathogens. We show that the YajQ homologue, CdgL (c-di-GMP receptor interacting with LysR) affects expression of the HSAF biosynthesis operon by interacting with the transcription activator LysR. The CdgL-LysR interaction enhances the apparent affinity of LysR to the promoter region upstream of the HSAF biosynthesis operon, which increases operon expression. Unlike the homologues CdgL (YajQ)-LysR system in X. campestris, we show that c-di-GMP binding to CdgL seems to weaken CdgL-LysR interactions and promote the release of CdgL from the LysR-DNA complex, which leads to decreased expression. Together, this study takes the YajQ-LysR-like system from bacterial pathogens to a crop-protecting bacterium that is able to regulate antifungal HSAF biosynthesis via disassembly of the c-di-GMP receptor-transcription activator complex. Published version 2020-06-18T02:46:45Z 2020-06-18T02:46:45Z 2020 Journal Article Han, S., Shen, D., Wang, Y.-C., Chou, S.-H., Gomelsky, M., Gao, Y.-G., & Qian, G. (2020). A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes. Molecular Plant Pathology, 21(2), 218-229. doi:10.1111/mpp.12890 1464-6722 https://hdl.handle.net/10356/142259 10.1111/mpp.12890 31747123 2-s2.0-85075247441 2 21 218 229 en Molecular Plant Pathology © 2019 The Authors. (published by British Society for Plant Pathology and John Wiley & Sons Ltd). This is an open access article under the terms of the Creative Commons Attribution NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Science::Biological sciences
Antifungal Antibiotic
CdgL
spellingShingle Science::Biological sciences
Antifungal Antibiotic
CdgL
Han, Sen
Shen, Danyu
Wang, Yu-Chuan
Chou, Shan-Ho
Gomelsky, Mark
Gao, Yong-Gui
Qian, Guoliang
A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
description YajQ, a binding protein of the universal bacterial second messenger cyclic di-GMP (c-di-GMP), affects virulence in several bacterial pathogens, including Xanthomonas campestris. In this bacterium, YajQ interacts with the transcription factor LysR. Upon c-di-GMP binding, the whole c-di-GMP-YajQ-LysR complex is found to dissociate from DNA, resulting in virulence gene regulation. Here, we identify a YajQ-LysR-like system in the bacterial biocontrol agent Lysobacter enzymogenes OH11 that secretes an antifungal antibiotic, heat-stable antifungal factor (HSAF) against crop fungal pathogens. We show that the YajQ homologue, CdgL (c-di-GMP receptor interacting with LysR) affects expression of the HSAF biosynthesis operon by interacting with the transcription activator LysR. The CdgL-LysR interaction enhances the apparent affinity of LysR to the promoter region upstream of the HSAF biosynthesis operon, which increases operon expression. Unlike the homologues CdgL (YajQ)-LysR system in X. campestris, we show that c-di-GMP binding to CdgL seems to weaken CdgL-LysR interactions and promote the release of CdgL from the LysR-DNA complex, which leads to decreased expression. Together, this study takes the YajQ-LysR-like system from bacterial pathogens to a crop-protecting bacterium that is able to regulate antifungal HSAF biosynthesis via disassembly of the c-di-GMP receptor-transcription activator complex.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Han, Sen
Shen, Danyu
Wang, Yu-Chuan
Chou, Shan-Ho
Gomelsky, Mark
Gao, Yong-Gui
Qian, Guoliang
format Article
author Han, Sen
Shen, Danyu
Wang, Yu-Chuan
Chou, Shan-Ho
Gomelsky, Mark
Gao, Yong-Gui
Qian, Guoliang
author_sort Han, Sen
title A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
title_short A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
title_full A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
title_fullStr A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
title_full_unstemmed A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes
title_sort yajq-lysr-like, cyclic di-gmp-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, lysobacter enzymogenes
publishDate 2020
url https://hdl.handle.net/10356/142259
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