A human expression system based on HEK293 for the stable production of recombinant erythropoietin

• Main Authors: Chin, Christine Lin, Goh, Justin Bryan, Srinivasan, Harini, Liu, Kaiwen Ivy, Gowher, Ali, Shanmugam, Raghuvaran, Lim, Hsueh Lee, Choo, Matthew, Tang, Wen Qin, Tan, Andy Hee-Meng, Nguyen-Khuong, Terry, Tan, Meng How, Ng, Say Kong
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Engineering::Chemical engineering; HEK293; Chinese Hamster Ovary (CHO)
• Online Access: https://hdl.handle.net/10356/142410
• Institution: Nanyang Technological University

Mammalian host cell lines are the preferred expression systems for the manufacture of complex therapeutics and recombinant proteins. However, the most utilized mammalian host systems, namely Chinese hamster ovary (CHO), Sp2/0 and NS0 mouse myeloma cells, can produce glycoproteins with non-human glyc...