A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells

• Main Authors: Siddiqui, Asim Azhar, Peter, Sabrina, Ngoh, Eve Zi Xian, Wang, Cheng-I, Ng, Shirelle, Dangerfield, John A., Gunzburg, Walter H., Dröge, Peter, Makhija, Harshyaa
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2023
• 主題: Science; Human Cell Line Engineering; λ Integrase; Expi293F Cells; Biotherapeutics; Site-Specific Transgenesis; Microencapsulation
• 在線閱讀: https://hdl.handle.net/10356/168601
• 機構: Nanyang Technological University

Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, a sequence-specific DNA recombinase, we developed a novel transgenesis platform involving a fully characterized single genomic locus as an ar...