A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells

• المؤلفون الرئيسيون: Siddiqui, Asim Azhar, Peter, Sabrina, Ngoh, Eve Zi Xian, Wang, Cheng-I, Ng, Shirelle, Dangerfield, John A., Gunzburg, Walter H., Dröge, Peter, Makhija, Harshyaa
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2023
• الموضوعات: Science; Human Cell Line Engineering; λ Integrase; Expi293F Cells; Biotherapeutics; Site-Specific Transgenesis; Microencapsulation
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/168601

Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, a sequence-specific DNA recombinase, we developed a novel transgenesis platform involving a fully characterized single genomic locus as an ar...