Can we use 2,3,5-Triphenyltetrazolium chloride-stained brain slices for other purposes? the application of western blotting

• Main Authors: Sanchez-Bezanilla, Sonia, Nilsson, Michael, Walker, Frederick Rohan, Ong, Lin Kooi
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Medicine; Stroke; 2,3,5-Triphenyltetrazolium Chloride
• Online Access: https://hdl.handle.net/10356/142446
• Institution: Nanyang Technological University

2,3,5-Triphenyltetrazolium chloride (TTC) staining is a commonly used method to determine the volume of the cerebral infarction in experimental stroke models. The TTC staining protocol is considered to interfere with downstream analyses, and it is unclear whether TTC-stained brain samples can be use...