AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring
RNA interference (RNAi) is demonstrated as one of the most powerful technologies for sequence-specific suppression of genes in disease therapeutics. Exploration of novel vehicles for small interfering RNA (siRNA) delivery with high efficiency, low cytotoxicity, and self-monitoring functionality is p...
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sg-ntu-dr.10356-1436582020-09-15T07:21:54Z AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring He, Xuewen Yin, Feng Wang, Dongyuan Xiong, Ling-Hong Kwok, Ryan T. K. Gao, Peng Fei Zhao, Zheng Lam, Jacky W. Y. Yong, Ken-Tye Li, Zigang Tang, Ben Zhong School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Aggregation-induced Emission Nanocarrier RNA interference (RNAi) is demonstrated as one of the most powerful technologies for sequence-specific suppression of genes in disease therapeutics. Exploration of novel vehicles for small interfering RNA (siRNA) delivery with high efficiency, low cytotoxicity, and self-monitoring functionality is persistently pursued. Herein, by taking advantage of aggregation-induced emission luminogen (AIEgen), we developed a novel class of Ag@AIE core@shell nanocarriers with regulable and uniform morphology. It presented excellent efficiencies in siRNA delivery, target gene knockdown, and cancer cell inhibition in vitro. What's more, an anticancer efficacy up to 75% was achieved in small animal experiments without obvious toxicity. Attributing to the unique AIE properties, real-time intracellular tracking of siRNA delivery and long-term tumor tissue imaging were successfully realized. Compared to the commercial transfection reagents, significant improvements were obtained in biocompatibility, delivery efficiency, and reproducibility, representing a promising future of this nanocarrier in RNAi-related cancer therapeutics. Accepted version The authors acknowledge funding to B.Z.T. from the Innovation and Technology Commission (ITCCNERC14SC01 and ITS/254117), the National Science Foundation of China (NSFC 21788102), and the Research Grants Council of Hong Kong (16305015, N_HKUST604/14, A-HKUST605/16, and C6009-17G). B.Z.T. is also grateful for the support from the Science and Technology Plan of Shenzhen (JCYJ20160229205601482). The authors also acknowledge funding to Z.L. (NSFC 1778009), F.Y. (NSFC 81701818), and L.-H.X. (NSFC 21705111). 2020-09-15T07:19:55Z 2020-09-15T07:19:55Z 2019 Journal Article He, X., Yin, F., Wang, D., Xiong, L.-H., Kwok, R. T. K., Gao, P. F., . . . Tang, B. Z. (2019).AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring. Nano Letters, 19(4), 2272-2279. doi:10.1021/acs.nanolett.8b04677 1530-6984 https://hdl.handle.net/10356/143658 10.1021/acs.nanolett.8b04677 30829039 4 19 2272 2279 en Nano Letters This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.nanolett.8b04677 application/pdf |
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Engineering::Electrical and electronic engineering Aggregation-induced Emission Nanocarrier He, Xuewen Yin, Feng Wang, Dongyuan Xiong, Ling-Hong Kwok, Ryan T. K. Gao, Peng Fei Zhao, Zheng Lam, Jacky W. Y. Yong, Ken-Tye Li, Zigang Tang, Ben Zhong AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
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RNA interference (RNAi) is demonstrated as one of the most powerful technologies for sequence-specific suppression of genes in disease therapeutics. Exploration of novel vehicles for small interfering RNA (siRNA) delivery with high efficiency, low cytotoxicity, and self-monitoring functionality is persistently pursued. Herein, by taking advantage of aggregation-induced emission luminogen (AIEgen), we developed a novel class of Ag@AIE core@shell nanocarriers with regulable and uniform morphology. It presented excellent efficiencies in siRNA delivery, target gene knockdown, and cancer cell inhibition in vitro. What's more, an anticancer efficacy up to 75% was achieved in small animal experiments without obvious toxicity. Attributing to the unique AIE properties, real-time intracellular tracking of siRNA delivery and long-term tumor tissue imaging were successfully realized. Compared to the commercial transfection reagents, significant improvements were obtained in biocompatibility, delivery efficiency, and reproducibility, representing a promising future of this nanocarrier in RNAi-related cancer therapeutics. |
author2 |
School of Electrical and Electronic Engineering |
author_facet |
School of Electrical and Electronic Engineering He, Xuewen Yin, Feng Wang, Dongyuan Xiong, Ling-Hong Kwok, Ryan T. K. Gao, Peng Fei Zhao, Zheng Lam, Jacky W. Y. Yong, Ken-Tye Li, Zigang Tang, Ben Zhong |
format |
Article |
author |
He, Xuewen Yin, Feng Wang, Dongyuan Xiong, Ling-Hong Kwok, Ryan T. K. Gao, Peng Fei Zhao, Zheng Lam, Jacky W. Y. Yong, Ken-Tye Li, Zigang Tang, Ben Zhong |
author_sort |
He, Xuewen |
title |
AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
title_short |
AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
title_full |
AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
title_fullStr |
AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
title_full_unstemmed |
AIE featured inorganic-organic Core@Shell nanoparticles for high-efficiency siRNA delivery and real-time monitoring |
title_sort |
aie featured inorganic-organic core@shell nanoparticles for high-efficiency sirna delivery and real-time monitoring |
publishDate |
2020 |
url |
https://hdl.handle.net/10356/143658 |
_version_ |
1681057210356989952 |