A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state

• Main Authors: Szczerbinska, Iwona, Gonzales, Kevin Andrew Uy, Cukuroglu, Engin, Muhammad Nadzim Bin Ramli, Lee, Bertha Pei Ge, Tan, Cheng Peow, Wong, Cheng Kit, Rancati, Giulia Irene, Liang, Hongqing, Göke, Jonathan, Ng, Huck-Hui, Chan, Yun-Shen
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Biological sciences; Feeder-free System; Naive Pluripotent State
• Online Access: https://hdl.handle.net/10356/143817
• Institution: Nanyang Technological University

The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols re...