Role of Lipopolysaccharide in protecting OmpT from autoproteolysis during in vitro refolding

• Main Authors: Sinsinbar, Gaurav, Gudlur, Sushanth, Metcalf, Kevin J., Mrksich, Milan, Nallani, Madhavan, Liedberg, Bo
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Biological sciences; LPS; Autoproteolysis
• Online Access: https://hdl.handle.net/10356/144253; https://doi.org/10.3390/biom10060922
• Institution: Nanyang Technological University

Outer membrane protease (OmpT) is a 33.5 kDa aspartyl protease that cleaves at dibasic sites and is thought to function as a defense mechanism for E. coli against cationic antimicrobial peptides secreted by the host immune system. Despite carrying three dibasic sites in its own sequence, there is no...