Rapid, sensitive and highly specific label-free fluorescence biosensor for microRNA by branched rolling circle amplification

• Main Authors: Ma, Qian, Li, Pin, Gao, Zhiqiang, Li, Sam Fong Yau
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Medicine; MicroRNA; Terpyridine Zinc
• Online Access: https://hdl.handle.net/10356/144341
• Institution: Nanyang Technological University

A simple fluorescence biosensor for rapid and sensitive target microRNA (miRNA) quantification by branched rolling circle amplification (BRCA) is developed in this work. Target miRNA functions as primer to recognize and hybridize with a circle DNA template, initiating rolling circle amplification (R...