Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA

The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are...

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Main Authors: Shi, Xiangyan, Prasanna, Chinmayi, Soman, Aghil, Pervushin, Konstantin, Nordenskiöld, Lars
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/145367
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spelling sg-ntu-dr.10356-1453672023-02-28T19:25:16Z Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA Shi, Xiangyan Prasanna, Chinmayi Soman, Aghil Pervushin, Konstantin Nordenskiöld, Lars School of Physical and Mathematical Sciences School of Biological Sciences Science::Biological sciences Biochemistry Biophysics The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes. Ministry of Education (MOE) Nanyang Technological University Published version We thank Dr. Nikolay Korolev for comments on the manuscript. This work was supported by the Singapore Ministry of Education Academic Research Fund (AcRF) Tier 2 (MOE2018-T2-1-112) and Tier 3 (MOE2012-T3-1-001). All SSNMR experiments were performed at the Nanyang Technological University (NTU) Center of High Field NMR Spectroscopy and Imaging. We also acknowledge the NTU Institute of Structural Biology (NISB) for supporting this research. 2020-12-18T07:53:10Z 2020-12-18T07:53:10Z 2020 Journal Article Shi, X., Prasanna, C., Soman, A., Pervushin, K., & Nordenskiöld, L. (2020). Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA. Communications Biology, 3(1), 639-. doi:10.1038/s42003-020-01369-3 2399-3642 https://hdl.handle.net/10356/145367 10.1038/s42003-020-01369-3 33128005 1 3 en MOE2018-T2-1-112 MOE2012-T3-1-001 Communications Biology © 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Biochemistry
Biophysics
spellingShingle Science::Biological sciences
Biochemistry
Biophysics
Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
description The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
format Article
author Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
author_sort Shi, Xiangyan
title Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_short Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_full Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_fullStr Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_full_unstemmed Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_sort dynamic networks observed in the nucleosome core particles couple the histone globular domains with dna
publishDate 2020
url https://hdl.handle.net/10356/145367
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