Defining essential enhancers for pluripotent stem cells using a features-oriented CRISPR-Cas9 screen

• Main Authors: Wang, Hao Fei, Warrier, Tushar, Farran, Chadi A., Zheng, Zi Hao, Xing, Qiao Rui, Fullwood, Melissa Jane, Zhang, Li-Feng, Li, Hu, Xu, Jian, Lim, Tit-Meng, Loh, Yuin-Han
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; CRISPR Screen; OCT4-bound
• Online Access: https://hdl.handle.net/10356/145979
• Institution: Nanyang Technological University

cis-regulatory elements (CREs) regulate the expression of genes in their genomic neighborhoods and influence cellular processes such as cell-fate maintenance and differentiation. To date, there remain major gaps in the functional characterization of CREs and the identification of their target genes...