Structural modelling of the lumenal domain of human GPAA1, the metallo-peptide synthetase subunit of the transamidase complex, reveals zinc-binding mode and two flaps surrounding the active site

• Main Authors: Su, Chinh Tran-To, Sinha, Swati, Eisenhaber, Birgit, Eisenhaber, Frank
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; GPI Lipid Anchoring; Transamidase
• Online Access: https://hdl.handle.net/10356/148368
• Institution: Nanyang Technological University

Background: The transamidase complex is a molecular machine in the endoplasmic reticulum of eukaryotes that attaches a glycosylphosphatidylinositol (GPI) lipid anchor to substrate proteins after cleaving a C-terminal propeptide with a defined sequence signal. Its five subunits are very hydrophobic;...