The eye as a transplantation site to monitor pancreatic islet cell plasticity

The endocrine cells confined in the islets of Langerhans are responsible for the maintenance of blood glucose homeostasis. In particular, beta cells produce and secrete insulin, an essential hormone regulating glucose uptake and metabolism. An insufficient amount of beta cells or defects in the mole...

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Main Authors: Ilegems, Erwin, Berggren, Per-Olof
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/152055
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spelling sg-ntu-dr.10356-1520552023-03-05T16:44:11Z The eye as a transplantation site to monitor pancreatic islet cell plasticity Ilegems, Erwin Berggren, Per-Olof Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Pancreatic Islet Imaging Islet Transplantation The endocrine cells confined in the islets of Langerhans are responsible for the maintenance of blood glucose homeostasis. In particular, beta cells produce and secrete insulin, an essential hormone regulating glucose uptake and metabolism. An insufficient amount of beta cells or defects in the molecular mechanisms leading to glucose-induced insulin secretion trigger the development of diabetes, a severe disease with epidemic spreading throughout the world. A comprehensive appreciation of the diverse adaptive procedures regulating beta cell mass and function is thus of paramount importance for the understanding of diabetes pathogenesis and for the development of effective therapeutic strategies. While significant findings were obtained by the use of islets isolated from the pancreas, in vitro studies are inherently limited since they lack the many factors influencing pancreatic islet cell function in vivo and do not allow for longitudinal monitoring of islet cell plasticity in the living organism. In this respect a number of imaging methodologies have been developed over the years for the study of islets in situ in the pancreas, a challenging task due to the relatively small size of the islets and their location, scattered throughout the organ. To increase imaging resolution and allow for longitudinal studies in individual islets, another strategy is based on the transplantation of islets into other sites that are more accessible for imaging. In this review we present the anterior chamber of the eye as a transplantation and imaging site for the study of pancreatic islet cell plasticity, and summarize the major research outcomes facilitated by this technological platform. Published version Own work discussed in this review was supported by funding from Karolinska Institutet, the Strategic Research Program in Diabetes at Karolinska Institutet, the Swedish Research Council, the Novo Nordisk Foundation, the Swedish Diabetes Association, the Family Knut and Alice Wallenberg Foundation, Diabetes Research and Wellness Foundation, the Stichting af Jochnick Foundation, the Family Erling-Persson Foundation, Berth von Kantzow’s Foundation, ERC-2018-AdG 834860 EYELETS, the European Union’s Seventh Framework Programme under grant agreements No 289932 and 613879, and the European Diabetes Research Programme in Cellular Plasticity Underlying the Pathophysiology of Type 2 Diabetes. 2021-11-18T06:26:41Z 2021-11-18T06:26:41Z 2021 Journal Article Ilegems, E. & Berggren, P. (2021). The eye as a transplantation site to monitor pancreatic islet cell plasticity. Frontiers in Endocrinology, 12, 652853-. https://dx.doi.org/10.3389/fendo.2021.652853 1664-2392 https://hdl.handle.net/10356/152055 10.3389/fendo.2021.652853 33967961 2-s2.0-85105454462 12 652853 en Frontiers in Endocrinology © 2021 Ilegems and Berggren. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Pancreatic Islet Imaging
Islet Transplantation
spellingShingle Science::Medicine
Pancreatic Islet Imaging
Islet Transplantation
Ilegems, Erwin
Berggren, Per-Olof
The eye as a transplantation site to monitor pancreatic islet cell plasticity
description The endocrine cells confined in the islets of Langerhans are responsible for the maintenance of blood glucose homeostasis. In particular, beta cells produce and secrete insulin, an essential hormone regulating glucose uptake and metabolism. An insufficient amount of beta cells or defects in the molecular mechanisms leading to glucose-induced insulin secretion trigger the development of diabetes, a severe disease with epidemic spreading throughout the world. A comprehensive appreciation of the diverse adaptive procedures regulating beta cell mass and function is thus of paramount importance for the understanding of diabetes pathogenesis and for the development of effective therapeutic strategies. While significant findings were obtained by the use of islets isolated from the pancreas, in vitro studies are inherently limited since they lack the many factors influencing pancreatic islet cell function in vivo and do not allow for longitudinal monitoring of islet cell plasticity in the living organism. In this respect a number of imaging methodologies have been developed over the years for the study of islets in situ in the pancreas, a challenging task due to the relatively small size of the islets and their location, scattered throughout the organ. To increase imaging resolution and allow for longitudinal studies in individual islets, another strategy is based on the transplantation of islets into other sites that are more accessible for imaging. In this review we present the anterior chamber of the eye as a transplantation and imaging site for the study of pancreatic islet cell plasticity, and summarize the major research outcomes facilitated by this technological platform.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Ilegems, Erwin
Berggren, Per-Olof
format Article
author Ilegems, Erwin
Berggren, Per-Olof
author_sort Ilegems, Erwin
title The eye as a transplantation site to monitor pancreatic islet cell plasticity
title_short The eye as a transplantation site to monitor pancreatic islet cell plasticity
title_full The eye as a transplantation site to monitor pancreatic islet cell plasticity
title_fullStr The eye as a transplantation site to monitor pancreatic islet cell plasticity
title_full_unstemmed The eye as a transplantation site to monitor pancreatic islet cell plasticity
title_sort eye as a transplantation site to monitor pancreatic islet cell plasticity
publishDate 2021
url https://hdl.handle.net/10356/152055
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