Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides
Zwitterionic peptides emerge as a class of highly effective antifouling materials in a wide range of applications such as biosensor, biomedical devices, and implants. We incorporated neutral amino acid spacers with different hydrophobicities, including serine (Ser), glycine (Gly), and leucine (Leu),...
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sg-ntu-dr.10356-1527332023-07-14T16:00:47Z Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides Li, Chuanxi Li, Minglun Qi, Wei Su, Rongxin Yu, Jing School of Materials Science and Engineering Science::Chemistry::Biochemistry Peptides and Proteins Monomers Zwitterionic peptides emerge as a class of highly effective antifouling materials in a wide range of applications such as biosensor, biomedical devices, and implants. We incorporated neutral amino acid spacers with different hydrophobicities, including serine (Ser), glycine (Gly), and leucine (Leu), into zwitterionic peptides with KE repeating units and investigated the structure and antifouling performance of the zwitterionic peptide brushes using surface plasma resonance (SPR), surface force apparatus (SFA), and all atomistic molecular dynamics (MD) simulation techniques. Our results demonstrate that the hydrophilicity of neutral spacers alters the structure and antifouling performance of the peptide-modified surface. Hydrophilic Ser inserted peptides reduced the interaction between the peptide monolayer and protein foulants while hydrophobic Leu significantly increased the protein adhesion. SFA force measurements shows that the presence of more spacers would increase the adhesion between the peptide monolayer and the modeling foulant lysozyme, especially for the hydrophobic spacers. MD simulations reveal that hydrophilic Ser spacers retain the hydrophilicity of the peptide monolayer and improve the antifouling performance, and Gly spacers give rise to more inter-chain crosslinks. Leu spacers result in a more hydrophobic peptide monolayer which lead to dehydration of the peptide monolayer and reduces the antifouling performances. Ministry of Education (MOE) National Research Foundation (NRF) Accepted version C.L. and R.S. acknowledge the National Natural Science Foundation of China (21621004), the Tianjin Municipal Science and Technology Bureau, China (16JCZDJC37900), the Ministry of Education (grant no. NCET-11-0372), and financial support from the China Scholarship Council (CSC, 201806250100). M.L. and J.Y. acknowledge the Singapore Ministry of Education Academic Research Fund Tier 1 (RG7/ 19) and the Singapore National Research Fellowship (NRFNRFF11-2019-0004). 2021-09-22T06:51:53Z 2021-09-22T06:51:53Z 2021 Journal Article Li, C., Li, M., Qi, W., Su, R. & Yu, J. (2021). Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides. Langmuir, 37(28), 8455-8462. https://dx.doi.org/10.1021/acs.langmuir.1c00803 0743-7463 https://hdl.handle.net/10356/152733 10.1021/acs.langmuir.1c00803 28 37 8455 8462 en RG7/19 NRF-NRFF11-2019-0004 Langmuir This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/http://doi.org/10.1021/acs.langmuir.1c00803. application/pdf |
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Science::Chemistry::Biochemistry Peptides and Proteins Monomers Li, Chuanxi Li, Minglun Qi, Wei Su, Rongxin Yu, Jing Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
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Zwitterionic peptides emerge as a class of highly effective antifouling materials in a wide range of applications such as biosensor, biomedical devices, and implants. We incorporated neutral amino acid spacers with different hydrophobicities, including serine (Ser), glycine (Gly), and leucine (Leu), into zwitterionic peptides with KE repeating units and investigated the structure and antifouling performance of the zwitterionic peptide brushes using surface plasma resonance (SPR), surface force apparatus (SFA), and all atomistic molecular dynamics (MD) simulation techniques. Our results demonstrate that the hydrophilicity of neutral spacers alters the structure and antifouling performance of the peptide-modified surface. Hydrophilic Ser inserted peptides reduced the interaction between the peptide monolayer and protein foulants while hydrophobic Leu significantly increased the protein adhesion. SFA force measurements shows that the presence of more spacers would increase the adhesion between the peptide monolayer and the modeling foulant lysozyme, especially for the hydrophobic spacers. MD simulations reveal that hydrophilic Ser spacers retain the hydrophilicity of the peptide monolayer and improve the antifouling performance, and Gly spacers give rise to more inter-chain crosslinks. Leu spacers result in a more hydrophobic peptide monolayer which lead to dehydration of the peptide monolayer and reduces the antifouling performances. |
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School of Materials Science and Engineering |
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School of Materials Science and Engineering Li, Chuanxi Li, Minglun Qi, Wei Su, Rongxin Yu, Jing |
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Article |
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Li, Chuanxi Li, Minglun Qi, Wei Su, Rongxin Yu, Jing |
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Li, Chuanxi |
title |
Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
title_short |
Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
title_full |
Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
title_fullStr |
Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
title_full_unstemmed |
Effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
title_sort |
effect of hydrophobicity and charge separation on the antifouling properties of surface-tethered zwitterionic peptides |
publishDate |
2021 |
url |
https://hdl.handle.net/10356/152733 |
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1773551199833817088 |