Extracellular matrix and cellular senescence in venous leg ulcers

High prevalence of non-healing chronic wounds contributes to a huge healthcare burden across the world. Early treatment interventions for non-healing wounds are vital. It was previously shown that accumulation of 15% or more of senescent cells in a chronic wound edge is an indicator that the wound i...

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Main Authors: Lim, Debbie X. E., Richards, Toby, Kanapathy, Muholan, Sudhaharan, Thankiah, Wright, Graham D., Phillips, Anthony R. J., Becker, David Lawrence
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2021
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Online Access:https://hdl.handle.net/10356/153791
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spelling sg-ntu-dr.10356-1537912023-03-05T16:50:34Z Extracellular matrix and cellular senescence in venous leg ulcers Lim, Debbie X. E. Richards, Toby Kanapathy, Muholan Sudhaharan, Thankiah Wright, Graham D. Phillips, Anthony R. J. Becker, David Lawrence Lee Kong Chian School of Medicine (LKCMedicine) Skin Research Institute of Singapore Agency for Science, Technology and Research (A*Star) Science::Medicine Biomarkers Diseases High prevalence of non-healing chronic wounds contributes to a huge healthcare burden across the world. Early treatment interventions for non-healing wounds are vital. It was previously shown that accumulation of 15% or more of senescent cells in a chronic wound edge is an indicator that the wound is unlikely to heal. However, determining the presence of senescent cells would require invasive procedures such as tissue biopsies to be taken. In this study, we found a strong correlation between decreased collagen area and presence of senescent cells in human chronic wounds i.e. venous leg ulcer (VLU), diabetic foot ulcer (DFU) and pressure ulcer (PRU). We also report that the lowest collagen levels were found in VLU patients less than 60 years of age, with a persistent wound of > 24 months. Elevated levels of senescent cells were also found in VLU of males. Second harmonic imaging of collagen at the edge of chronic wounds with a handheld multiphoton device could be used to predict the number of senescent cells, indicating if the wound is on a healing trajectory or not. Our data support the use of collagen imaging in cutaneous wound assessment for a faster and non-invasive method to predict cellular senescence and determining wound trajectory of healing. Agency for Science, Technology and Research (A*STAR) National Research Foundation (NRF) Published version This research is supported by the Agency for Science, Technology and Research (A*STAR) under its Industry alignment fund—Pre-Positioning Programme (IAF-PP) with grant numbers H17/01/a0/0C9 as part of the Wound Care Innovation for the Tropics (WCIT) programme, sub awards H18/01/a0/019 and H1701a0004. The Skin Research Institute of Singapore, Phase 2: SRIS@Novena. AMP is funded by Singapore’s Agency for Science, Technology & Research (A*STAR) through core funds and under the HBMS IAF-PP Project (H1701a0004) and through the National Research Foundation Singapore under its Shared Infrastructure Support grant for SingaScope—a Singapore-wide microscopy infrastructure network (NRF2017_SISFP10)”. 2021-12-29T01:34:26Z 2021-12-29T01:34:26Z 2021 Journal Article Lim, D. X. E., Richards, T., Kanapathy, M., Sudhaharan, T., Wright, G. D., Phillips, A. R. J. & Becker, D. L. (2021). Extracellular matrix and cellular senescence in venous leg ulcers. Scientific Reports, 11(1), 20168-. https://dx.doi.org/10.1038/s41598-021-99643-9 2045-2322 https://hdl.handle.net/10356/153791 10.1038/s41598-021-99643-9 34635751 2-s2.0-85116823020 1 11 20168 en H17/01/a0/0C9 H18/01/a0/019 H1701a0004 H1701a0004 NRF2017_SISFP10 Scientific Reports © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Biomarkers
Diseases
spellingShingle Science::Medicine
Biomarkers
Diseases
Lim, Debbie X. E.
Richards, Toby
Kanapathy, Muholan
Sudhaharan, Thankiah
Wright, Graham D.
Phillips, Anthony R. J.
Becker, David Lawrence
Extracellular matrix and cellular senescence in venous leg ulcers
description High prevalence of non-healing chronic wounds contributes to a huge healthcare burden across the world. Early treatment interventions for non-healing wounds are vital. It was previously shown that accumulation of 15% or more of senescent cells in a chronic wound edge is an indicator that the wound is unlikely to heal. However, determining the presence of senescent cells would require invasive procedures such as tissue biopsies to be taken. In this study, we found a strong correlation between decreased collagen area and presence of senescent cells in human chronic wounds i.e. venous leg ulcer (VLU), diabetic foot ulcer (DFU) and pressure ulcer (PRU). We also report that the lowest collagen levels were found in VLU patients less than 60 years of age, with a persistent wound of > 24 months. Elevated levels of senescent cells were also found in VLU of males. Second harmonic imaging of collagen at the edge of chronic wounds with a handheld multiphoton device could be used to predict the number of senescent cells, indicating if the wound is on a healing trajectory or not. Our data support the use of collagen imaging in cutaneous wound assessment for a faster and non-invasive method to predict cellular senescence and determining wound trajectory of healing.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Lim, Debbie X. E.
Richards, Toby
Kanapathy, Muholan
Sudhaharan, Thankiah
Wright, Graham D.
Phillips, Anthony R. J.
Becker, David Lawrence
format Article
author Lim, Debbie X. E.
Richards, Toby
Kanapathy, Muholan
Sudhaharan, Thankiah
Wright, Graham D.
Phillips, Anthony R. J.
Becker, David Lawrence
author_sort Lim, Debbie X. E.
title Extracellular matrix and cellular senescence in venous leg ulcers
title_short Extracellular matrix and cellular senescence in venous leg ulcers
title_full Extracellular matrix and cellular senescence in venous leg ulcers
title_fullStr Extracellular matrix and cellular senescence in venous leg ulcers
title_full_unstemmed Extracellular matrix and cellular senescence in venous leg ulcers
title_sort extracellular matrix and cellular senescence in venous leg ulcers
publishDate 2021
url https://hdl.handle.net/10356/153791
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