Human platelet lysate as a replacement for fetal bovine serum in human corneal stromal keratocyte and fibroblast culture

• Main Authors: Seidelmann, Nina, Duarte Campos, Daniela F., Rohde, Malena, Johnen, Sandra, Salla, Sabine, Yam, Gary Hin-Fai, Mehta, Jodhbir Singh, Walter, Peter, Fuest, Matthias
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Materials; Cell Culture; Cornea
• Online Access: https://hdl.handle.net/10356/154013
• Institution: Nanyang Technological University

The isolation and propagation of primary human corneal stromal keratocytes (CSK) are crucial for cellular research and corneal tissue engineering. However, this delicate cell type easily transforms into stromal fibroblasts (SF) and scar inducing myofibroblasts (Myo-SF). Current protocols mainly rely...