Discovery, engineering and functional study of asparagine-specific ligases from plants

• Main Author: Chen, Yu
• Other Authors: James P Tam
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2022
• Subjects: Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/155533
• Institution: Nanyang Technological University

Asparaginyl endopeptidases (AEPs) are cysteine proteases cutting peptide bonds after Asx (Asn/Asp). Peptide Asx-specific ligases (PALs) are AEP-like peptide ligases reversing the proteolysis of AEPs and making peptide bonds at the Asx sites. Peptide ligases have considerable potentials in biotechnol...