Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is...
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| Format: | Final Year Project |
| Language: | English |
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Nanyang Technological University
2022
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| Online Access: | https://hdl.handle.net/10356/158716 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is done using SDS to determine which is a better surfactant for decellularization, Triton X-100 or SDS. |
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