Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus

The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is...

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Main Author: S. Ramiyaasri
Other Authors: Chian Kerm Sin
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2022
Subjects:
Online Access:https://hdl.handle.net/10356/158716
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1587162023-03-04T20:07:02Z Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus S. Ramiyaasri Chian Kerm Sin School of Mechanical and Aerospace Engineering ASKSChian@ntu.edu.sg Engineering::Mechanical engineering The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is done using SDS to determine which is a better surfactant for decellularization, Triton X-100 or SDS. Bachelor of Engineering (Mechanical Engineering) 2022-06-06T07:20:22Z 2022-06-06T07:20:22Z 2022 Final Year Project (FYP) S. Ramiyaasri (2022). Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/158716 https://hdl.handle.net/10356/158716 en B058 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Mechanical engineering
spellingShingle Engineering::Mechanical engineering
S. Ramiyaasri
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
description The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is done using SDS to determine which is a better surfactant for decellularization, Triton X-100 or SDS.
author2 Chian Kerm Sin
author_facet Chian Kerm Sin
S. Ramiyaasri
format Final Year Project
author S. Ramiyaasri
author_sort S. Ramiyaasri
title Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
title_short Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
title_full Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
title_fullStr Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
title_full_unstemmed Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
title_sort optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
publisher Nanyang Technological University
publishDate 2022
url https://hdl.handle.net/10356/158716
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