Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus
The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is...
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2022
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sg-ntu-dr.10356-1587162023-03-04T20:07:02Z Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus S. Ramiyaasri Chian Kerm Sin School of Mechanical and Aerospace Engineering ASKSChian@ntu.edu.sg Engineering::Mechanical engineering The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is done using SDS to determine which is a better surfactant for decellularization, Triton X-100 or SDS. Bachelor of Engineering (Mechanical Engineering) 2022-06-06T07:20:22Z 2022-06-06T07:20:22Z 2022 Final Year Project (FYP) S. Ramiyaasri (2022). Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/158716 https://hdl.handle.net/10356/158716 en B058 application/pdf Nanyang Technological University |
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Engineering::Mechanical engineering S. Ramiyaasri Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
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The report discuss the effects of Triton X-100 on the decellularization of porcine oesophagi. Then the optimal concentration of Triton X-100 is identified after analysing via visual examination, histology study and DNA quantification. With the optimal concentration identified, the same experiment is done using SDS to determine which is a better surfactant for decellularization, Triton X-100 or SDS. |
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Chian Kerm Sin |
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Chian Kerm Sin S. Ramiyaasri |
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Final Year Project |
author |
S. Ramiyaasri |
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S. Ramiyaasri |
title |
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
title_short |
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
title_full |
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
title_fullStr |
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
title_full_unstemmed |
Optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
title_sort |
optimisation of surfactant concentration and treatment time on decellularization and characterization of porcine oesophagus |
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Nanyang Technological University |
publishDate |
2022 |
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https://hdl.handle.net/10356/158716 |
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1759857784155799552 |