Characterisation of cell traction force in micropatterned tissues
Tissue engineering is a field of medical science that researches for alternative methods to promote the regeneration of damaged cells and the creation of new tissues. As many medical treatments are performed in vivo, it is difficult to analyse the cellular activities occurring within the body. Th...
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Format: | Final Year Project |
Language: | English |
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Nanyang Technological University
2022
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Online Access: | https://hdl.handle.net/10356/159129 |
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Institution: | Nanyang Technological University |
Language: | English |
Summary: | Tissue engineering is a field of medical science that researches for alternative methods
to promote the regeneration of damaged cells and the creation of new tissues. As many
medical treatments are performed in vivo, it is difficult to analyse the cellular activities
occurring within the body. Therefore, in vitro model is introduced in tissue engineering
to understand the behavioural change of a cell in an environment outside of the living
organism.
By analysing micropatterned tissue, the relationship between the shape pattern and the
cell-to-cell interactions will be examined. Traction force microscopy (TFM) is a
technique used in tissue engineering to calculate the traction force in cellular activities
from the displacement of fluorescence beads. As cell-to-cell interactions require
traction forces, performing TFM on micropatterned tissue would provide quantitative
traction forces value for the interpretation of patterned cell behaviour.
A circular pair pattern and an elongated shape pattern were designed in this experiment.
After conducting TFM on the different pattern designs, it was revealed that the traction
forces were exerted on the boundary of the furthest edge. From the trend of the traction
force result, the phenomenon of cell dipole was introduced as a hypothesis for the
cause of the trend. The circular pair pattern resulted in the formation of a cell bridge
and was compared with the elongated pattern to understand how different designs
affect the traction forces. Lastly, modifications and recommendations for this research
are subsequently discussed. |
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