Characterisation of cell traction force in micropatterned tissues

Tissue engineering is a field of medical science that researches for alternative methods to promote the regeneration of damaged cells and the creation of new tissues. As many medical treatments are performed in vivo, it is difficult to analyse the cellular activities occurring within the body. Th...

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Main Author: Cheng, Yu Jun
Other Authors: Huang Changjin
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2022
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Online Access:https://hdl.handle.net/10356/159129
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1591292023-03-04T20:10:41Z Characterisation of cell traction force in micropatterned tissues Cheng, Yu Jun Huang Changjin K Jimmy Hsia School of Mechanical and Aerospace Engineering cjhuang@ntu.edu.sg, kjhsia@ntu.edu.sg Engineering::Mechanical engineering Tissue engineering is a field of medical science that researches for alternative methods to promote the regeneration of damaged cells and the creation of new tissues. As many medical treatments are performed in vivo, it is difficult to analyse the cellular activities occurring within the body. Therefore, in vitro model is introduced in tissue engineering to understand the behavioural change of a cell in an environment outside of the living organism. By analysing micropatterned tissue, the relationship between the shape pattern and the cell-to-cell interactions will be examined. Traction force microscopy (TFM) is a technique used in tissue engineering to calculate the traction force in cellular activities from the displacement of fluorescence beads. As cell-to-cell interactions require traction forces, performing TFM on micropatterned tissue would provide quantitative traction forces value for the interpretation of patterned cell behaviour. A circular pair pattern and an elongated shape pattern were designed in this experiment. After conducting TFM on the different pattern designs, it was revealed that the traction forces were exerted on the boundary of the furthest edge. From the trend of the traction force result, the phenomenon of cell dipole was introduced as a hypothesis for the cause of the trend. The circular pair pattern resulted in the formation of a cell bridge and was compared with the elongated pattern to understand how different designs affect the traction forces. Lastly, modifications and recommendations for this research are subsequently discussed. Bachelor of Engineering (Mechanical Engineering) 2022-06-10T04:08:35Z 2022-06-10T04:08:35Z 2022 Final Year Project (FYP) Cheng, Y. J. (2022). Characterisation of cell traction force in micropatterned tissues. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/159129 https://hdl.handle.net/10356/159129 en A204 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Mechanical engineering
spellingShingle Engineering::Mechanical engineering
Cheng, Yu Jun
Characterisation of cell traction force in micropatterned tissues
description Tissue engineering is a field of medical science that researches for alternative methods to promote the regeneration of damaged cells and the creation of new tissues. As many medical treatments are performed in vivo, it is difficult to analyse the cellular activities occurring within the body. Therefore, in vitro model is introduced in tissue engineering to understand the behavioural change of a cell in an environment outside of the living organism. By analysing micropatterned tissue, the relationship between the shape pattern and the cell-to-cell interactions will be examined. Traction force microscopy (TFM) is a technique used in tissue engineering to calculate the traction force in cellular activities from the displacement of fluorescence beads. As cell-to-cell interactions require traction forces, performing TFM on micropatterned tissue would provide quantitative traction forces value for the interpretation of patterned cell behaviour. A circular pair pattern and an elongated shape pattern were designed in this experiment. After conducting TFM on the different pattern designs, it was revealed that the traction forces were exerted on the boundary of the furthest edge. From the trend of the traction force result, the phenomenon of cell dipole was introduced as a hypothesis for the cause of the trend. The circular pair pattern resulted in the formation of a cell bridge and was compared with the elongated pattern to understand how different designs affect the traction forces. Lastly, modifications and recommendations for this research are subsequently discussed.
author2 Huang Changjin
author_facet Huang Changjin
Cheng, Yu Jun
format Final Year Project
author Cheng, Yu Jun
author_sort Cheng, Yu Jun
title Characterisation of cell traction force in micropatterned tissues
title_short Characterisation of cell traction force in micropatterned tissues
title_full Characterisation of cell traction force in micropatterned tissues
title_fullStr Characterisation of cell traction force in micropatterned tissues
title_full_unstemmed Characterisation of cell traction force in micropatterned tissues
title_sort characterisation of cell traction force in micropatterned tissues
publisher Nanyang Technological University
publishDate 2022
url https://hdl.handle.net/10356/159129
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