Quantitative structure-retention relationship for reliable metabolite identification and quantification in metabolomics using ion-pair reversed-phase chromatography coupled with tandem mass spectrometry

• Main Authors: Hu, Qingyu, Sun, Yuting, Yuan, Peihong, Lei, Hehua, Zhong, Huiqin, Wang, Yulan, Tang, Huiru
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; UHPLC-MS; Metabolomics
• Online Access: https://hdl.handle.net/10356/159581
• Institution: Nanyang Technological University

Hydrophilic metabolites are essential for all biological systems with multiple functions and their quantitative analysis forms an important part of metabolomics. However, poor retention of these metabolites on reversed-phase (RP) chromatographic column hinders their effective analysis with RPLC-MS m...