Manipulation of self-assembled three-dimensional architecture in reusable acoustofluidic device

• Main Authors: Nguyen, Tan Dai, Tran, Van-Thai, Du, Hejun
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2022
• Subjects: Engineering::Mechanical engineering; Acoustofluidics; 3D Patterning
• Online Access: https://hdl.handle.net/10356/159963
• Institution: Nanyang Technological University

Reconstructing of cell architecture plays a vital role in tissue engineering. Recent developments of self-assembling of cells into three-dimensional (3D) matrix pattern using surface acoustic waves have paved a way for a better tissue engineering platform thanks to its unique properties such as nature of noninvasive and noncontact, high biocompatibility, low-power consumption, automation capability, and fast actuation. This article discloses a method to manipulate the orientation and curvature of 3D matrix pattern by redesigning the top wall of microfluidic chamber and the technique to create a 3D longitudinal pattern along preinserted polydimethylsiloxane (PDMS) rods. Experimental results showed a good agreement with model predictions. This research can actively contribute to the development of better organs-on-chips platforms with capability of controlling cell architecture and density. Meanwhile, the 3D longitudinal pattern is suitable for self-assembling of microvasculatures.