Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction
Here we report a molecular docking-based approach to identify small molecules that can target the β-catenin (β-cat)-TCF4 protein-protein interaction (PPI), a key effector complex for nuclear Wnt signaling activity. Specifically, we developed and optimized a computational model of β-cat using publicl...
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sg-ntu-dr.10356-1606822023-02-28T17:11:53Z Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction Low, Joo-Leng Du, Weina Gocha, Tenzin Oguz, Gokce Zhang, Xiaoqian Chen, Ming Wei Masirevic, Srdan Yim, Daniel Guo Rong Tan, Iain Bee Huat Ramasamy, Adaikalavan Fan, Hao DasGupta, Ramanuj School of Biological Sciences BioSciences Research Centre Science::Biological sciences Biochemistry Cancer Here we report a molecular docking-based approach to identify small molecules that can target the β-catenin (β-cat)-TCF4 protein-protein interaction (PPI), a key effector complex for nuclear Wnt signaling activity. Specifically, we developed and optimized a computational model of β-cat using publicly available β-cat protein crystal structures, and existing β-cat-TCF4 interaction inhibitors as the training set. Using our computational model to an in silico screen predicted 27 compounds as good binders to β-cat, of which 3 were identified to be effective against a Wnt-responsive luciferase reporter. In vitro functional validation experiments revealed GB1874 as an inhibitor of the Wnt pathway that targets the β-cat-TCF4 PPI. GB1874 also affected the proliferation and stemness of Wnt-addicted colorectal cancer (CRC) cells in vitro. Encouragingly, GB1874 inhibited the growth of CRC tumor xenografts in vivo, thus demonstrating its potential for further development into therapeutics against Wnt-associated cancer indications. Published version R.D.G. was supported in part by NIH/NCI #1R01CA155125-01, and by the Genome Institute of Singapore (GIS) core funds (BMRC/A*STAR). H.F. was supported by the Bioinformatics Institute (BII) core funds (BMRC/A*STAR). J.-L.L. was supported by the National Research Foundation (NRF), Singapore Competitive Research Program (CRP) grant (NRF-CRP-2017-02). 2022-08-01T02:01:00Z 2022-08-01T02:01:00Z 2021 Journal Article Low, J., Du, W., Gocha, T., Oguz, G., Zhang, X., Chen, M. W., Masirevic, S., Yim, D. G. R., Tan, I. B. H., Ramasamy, A., Fan, H. & DasGupta, R. (2021). Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction. IScience, 24(6), 102544-. https://dx.doi.org/10.1016/j.isci.2021.102544 2589-0042 https://hdl.handle.net/10356/160682 10.1016/j.isci.2021.102544 34142050 2-s2.0-85107127642 6 24 102544 en iScience © 2021 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
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Science::Biological sciences Biochemistry Cancer Low, Joo-Leng Du, Weina Gocha, Tenzin Oguz, Gokce Zhang, Xiaoqian Chen, Ming Wei Masirevic, Srdan Yim, Daniel Guo Rong Tan, Iain Bee Huat Ramasamy, Adaikalavan Fan, Hao DasGupta, Ramanuj Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
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Here we report a molecular docking-based approach to identify small molecules that can target the β-catenin (β-cat)-TCF4 protein-protein interaction (PPI), a key effector complex for nuclear Wnt signaling activity. Specifically, we developed and optimized a computational model of β-cat using publicly available β-cat protein crystal structures, and existing β-cat-TCF4 interaction inhibitors as the training set. Using our computational model to an in silico screen predicted 27 compounds as good binders to β-cat, of which 3 were identified to be effective against a Wnt-responsive luciferase reporter. In vitro functional validation experiments revealed GB1874 as an inhibitor of the Wnt pathway that targets the β-cat-TCF4 PPI. GB1874 also affected the proliferation and stemness of Wnt-addicted colorectal cancer (CRC) cells in vitro. Encouragingly, GB1874 inhibited the growth of CRC tumor xenografts in vivo, thus demonstrating its potential for further development into therapeutics against Wnt-associated cancer indications. |
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School of Biological Sciences |
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School of Biological Sciences Low, Joo-Leng Du, Weina Gocha, Tenzin Oguz, Gokce Zhang, Xiaoqian Chen, Ming Wei Masirevic, Srdan Yim, Daniel Guo Rong Tan, Iain Bee Huat Ramasamy, Adaikalavan Fan, Hao DasGupta, Ramanuj |
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Article |
author |
Low, Joo-Leng Du, Weina Gocha, Tenzin Oguz, Gokce Zhang, Xiaoqian Chen, Ming Wei Masirevic, Srdan Yim, Daniel Guo Rong Tan, Iain Bee Huat Ramasamy, Adaikalavan Fan, Hao DasGupta, Ramanuj |
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Low, Joo-Leng |
title |
Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
title_short |
Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
title_full |
Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
title_fullStr |
Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
title_full_unstemmed |
Molecular docking-aided identification of small molecule inhibitors targeting β-catenin-TCF4 interaction |
title_sort |
molecular docking-aided identification of small molecule inhibitors targeting β-catenin-tcf4 interaction |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/160682 |
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1759857644787466240 |