Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering
Despite being a rich source of bioactive compounds, the current exploitation of aquatic biomass is insufficient. Majority of the aquaculture industry side-streams are currently used for low-value purposes such as animal feed or composting material, with low economical returns. To maximize resource r...
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sg-ntu-dr.10356-1607392022-08-02T02:15:07Z Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering Wang, Jun Kit Cimenoglu, Cigdem Cheam, Nicole Mein Ji Hu, Xiao Tay, Chor Yong School of Materials Science and Engineering School of Biological Sciences Nanyang Environment and Water Research Institute Energy Research Institute @ NTU (ERI@N) Engineering::Materials Waste Valorization Green Manufacturing Despite being a rich source of bioactive compounds, the current exploitation of aquatic biomass is insufficient. Majority of the aquaculture industry side-streams are currently used for low-value purposes such as animal feed or composting material, with low economical returns. To maximize resource reuse and minimize waste generation, valorization efforts should be augmented with the aim to produce high-value products. Herein, we present a novel aquaculture wastes-derived multi-scale osteoconductive hybrid biocomposite that is composed of chemically crosslinked American bullfrog (Rana catesbeiana) skin-derived type I tropocollagen nanofibrils (~22.3 nm) network and functionalized with micronized (~1.6 μm) single-phase hydroxyapatite (HA) from discarded snakehead (Channa micropeltes) fish scales. The bioengineered construct is biocompatible, highly porous (>90%), and exhibits excellent osteoconductive properties, as indicated by robust adhesion and proliferation of human fetal osteoblastic 1.19 cell line (hFOB 1.19). Furthermore, increased expression level of osteo-related ALPL and BGLAP mRNA transcripts, as well as enhanced osteocalcin immunoreactivity and increasing Alizarin red S staining coverage on the hybrid biocomposite was observed over 21 days of culture. Collectively, the devised "waste-to-resource" platform represents a sustainable waste valorization strategy that is amendable for advanced bone repair and regeneration applications. Ministry of Education (MOE) The authors gratefully acknowledge support from the Singapore Ministry of Education (MOE) Academic Research Fund Tier 1 (RG38/20 2020T1001152). 2022-08-02T02:15:07Z 2022-08-02T02:15:07Z 2021 Journal Article Wang, J. K., Cimenoglu, C., Cheam, N. M. J., Hu, X. & Tay, C. Y. (2021). Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering. Materials Science and Engineering: C, 126, 112104-. https://dx.doi.org/10.1016/j.msec.2021.112104 0928-4931 https://hdl.handle.net/10356/160739 10.1016/j.msec.2021.112104 34082928 2-s2.0-85105252881 126 112104 en RG38/20 2020-T1-001-152 Materials Science and Engineering: C © 2021 Elsevier B.V. All rights reserved. |
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Engineering::Materials Waste Valorization Green Manufacturing Wang, Jun Kit Cimenoglu, Cigdem Cheam, Nicole Mein Ji Hu, Xiao Tay, Chor Yong Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
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Despite being a rich source of bioactive compounds, the current exploitation of aquatic biomass is insufficient. Majority of the aquaculture industry side-streams are currently used for low-value purposes such as animal feed or composting material, with low economical returns. To maximize resource reuse and minimize waste generation, valorization efforts should be augmented with the aim to produce high-value products. Herein, we present a novel aquaculture wastes-derived multi-scale osteoconductive hybrid biocomposite that is composed of chemically crosslinked American bullfrog (Rana catesbeiana) skin-derived type I tropocollagen nanofibrils (~22.3 nm) network and functionalized with micronized (~1.6 μm) single-phase hydroxyapatite (HA) from discarded snakehead (Channa micropeltes) fish scales. The bioengineered construct is biocompatible, highly porous (>90%), and exhibits excellent osteoconductive properties, as indicated by robust adhesion and proliferation of human fetal osteoblastic 1.19 cell line (hFOB 1.19). Furthermore, increased expression level of osteo-related ALPL and BGLAP mRNA transcripts, as well as enhanced osteocalcin immunoreactivity and increasing Alizarin red S staining coverage on the hybrid biocomposite was observed over 21 days of culture. Collectively, the devised "waste-to-resource" platform represents a sustainable waste valorization strategy that is amendable for advanced bone repair and regeneration applications. |
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School of Materials Science and Engineering |
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School of Materials Science and Engineering Wang, Jun Kit Cimenoglu, Cigdem Cheam, Nicole Mein Ji Hu, Xiao Tay, Chor Yong |
format |
Article |
author |
Wang, Jun Kit Cimenoglu, Cigdem Cheam, Nicole Mein Ji Hu, Xiao Tay, Chor Yong |
author_sort |
Wang, Jun Kit |
title |
Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
title_short |
Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
title_full |
Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
title_fullStr |
Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
title_full_unstemmed |
Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
title_sort |
sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/160739 |
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1743119533065895936 |