Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum
Plasmodium falciparum (P. falciparum) is a protozoan parasite with the highest rates of mortality and most prevalent on the African continent. Given the emergence of antimalarial drug resistance in the recent years, the exact mechanisms of how the mutations or upregulation in the parasite increased...
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sg-ntu-dr.10356-1609972023-02-28T18:09:48Z Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum Toh, Victoria Le Yi Zbynek Bozdech School of Biological Sciences Josephine Boentoro ZBozdech@ntu.edu.sg Science::Biological sciences Plasmodium falciparum (P. falciparum) is a protozoan parasite with the highest rates of mortality and most prevalent on the African continent. Given the emergence of antimalarial drug resistance in the recent years, the exact mechanisms of how the mutations or upregulation in the parasite increased resistance against antimalarial drugs are still unclear. Previous methods to investigate Cyclophilin19B (CYP19B) as an over expression marker were used, therefore to further add on to this area of research, the possible location of CYP19B within P.falciparum is studied as well as its effect on artemisinin through western blotting, immunofluorescence and competition assays. It was interesting to note, through microscopy that CYP19B follows a similar protein localisation as the BiP protein within P.falciparum. Through the competition assay, it was inconclusive to determine that CYP19B aids in artemisinin resistance as there were no significant change in the prevalence of the overexpression plasmid in a mixed population. Nevertheless, this study proves that CYP19B is being over expressed and has some functions in the endoplasmic reticulum, corroborating well with current evidence regarding its role in protein folding. Hence, this knowledge can aid in helping understand the mechanism of artemisinin resistance Plasmodium parasites. Bachelor of Medicine (Chinese Medicine) Bachelor of Science in Biomedical Sciences 2022-08-11T06:01:04Z 2022-08-11T06:01:04Z 2022 Final Year Project (FYP) Toh, V. L. Y. (2022). Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/160997 https://hdl.handle.net/10356/160997 en application/pdf Nanyang Technological University |
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Science::Biological sciences Toh, Victoria Le Yi Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
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Plasmodium falciparum (P. falciparum) is a protozoan parasite with the highest rates of mortality and most prevalent on the African continent. Given the emergence of antimalarial drug resistance in the recent years, the exact mechanisms of how the mutations or upregulation in the parasite increased resistance against antimalarial drugs are still unclear. Previous methods to investigate Cyclophilin19B (CYP19B) as an over expression marker were used, therefore to further add on to this area of research, the possible location of CYP19B within P.falciparum is studied as well as its effect on artemisinin through western blotting, immunofluorescence and competition assays. It was interesting to note, through microscopy that CYP19B follows a similar protein localisation as the BiP protein within P.falciparum. Through the competition assay, it was inconclusive to determine that CYP19B aids in artemisinin resistance as there were no significant change in the prevalence of the overexpression plasmid in a mixed population. Nevertheless, this study proves that CYP19B is being over expressed and has some functions in the endoplasmic reticulum, corroborating well with current evidence regarding its role in protein folding. Hence, this knowledge can aid in helping understand the mechanism of artemisinin resistance Plasmodium parasites. |
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Zbynek Bozdech |
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Zbynek Bozdech Toh, Victoria Le Yi |
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Final Year Project |
author |
Toh, Victoria Le Yi |
author_sort |
Toh, Victoria Le Yi |
title |
Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
title_short |
Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
title_full |
Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
title_fullStr |
Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
title_full_unstemmed |
Characterizing the role of Cyp19B as a contributing factor to artemisinin resistance in Plasmodium falciparum |
title_sort |
characterizing the role of cyp19b as a contributing factor to artemisinin resistance in plasmodium falciparum |
publisher |
Nanyang Technological University |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/160997 |
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1759853574116868096 |