Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers
Sapindus is an important forest tree genus with utilization in biodiesel, biomedicine, and it harbors great potential for biochemical engineering applications. For advanced breeding of Sapindus, it is necessary to evaluate the genetic diversity and construct a rationally designed core germplasm coll...
Saved in:
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/161052 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-161052 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1610522023-02-28T17:13:26Z Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers Liu, Jiming Gao, Shilun Xu, Yuanyuan Wang, Mianzhi Ngiam, Jia Jun Cho, Nicholas Rui Wen Jong, Joan Jing Yi Weng, Xuehuang Jia, Liming Salojärvi, Jarkko School of Biological Sciences Science::Biological sciences Genetic Diversity Population Structure Sapindus is an important forest tree genus with utilization in biodiesel, biomedicine, and it harbors great potential for biochemical engineering applications. For advanced breeding of Sapindus, it is necessary to evaluate the genetic diversity and construct a rationally designed core germplasm collection. In this study, the genetic diversity and population structure of Sapindus were conducted with 18 expressed sequence tag-simple sequence repeat (EST-SSR) markers in order to establish a core germplasm collection from 161 Sapindus accessions. The population of Sapindus showed high genetic diversity and significant population structure. Interspecific genetic variation was significantly higher than intraspecific variation in the Sapindus mukorossi, Sapindus delavayi, and combined Sapindus rarak plus Sapindus rarak var. velutinus populations. S. mukorossi had abundant genetic variation and showed a specific pattern of geographical variation, whereas S. delavayi, S. rarak, and S. rarak var. velutinus showed less intraspecific variation. A core germplasm collection was created that contained 40% of genetic variation in the initial population, comprising 53 S. mukorossi and nine S. delavayi lineages, as well as single representatives of S. rarak and S. rarak var. velutinus. These results provide a germplasm basis and theoretical rationale for the efficient management, conservation, and utilization of Sapindus, as well as genetic resources for joint genomics research in the future. Published version This work was supported by Special Foundation for National Science and Technology Basic Research Program of China (No. 2019FY100803), the National Natural Science Foundation of China (32071793), the China Scholarship Council, and the Academy of Finland (decisions 346100 and 319947). 2022-08-12T07:52:28Z 2022-08-12T07:52:28Z 2022 Journal Article Liu, J., Gao, S., Xu, Y., Wang, M., Ngiam, J. J., Cho, N. R. W., Jong, J. J. Y., Weng, X., Jia, L. & Salojärvi, J. (2022). Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers. Frontiers in Plant Science, 13, 857993-. https://dx.doi.org/10.3389/fpls.2022.857993 1664-462X https://hdl.handle.net/10356/161052 10.3389/fpls.2022.857993 35685004 2-s2.0-85131875794 13 857993 en Frontiers in Plant Science © 2022 Liu, Gao, Xu, Wang, Ngiam, Rui Wen, Yi, Weng, Jia and Salojärvi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Science::Biological sciences Genetic Diversity Population Structure |
spellingShingle |
Science::Biological sciences Genetic Diversity Population Structure Liu, Jiming Gao, Shilun Xu, Yuanyuan Wang, Mianzhi Ngiam, Jia Jun Cho, Nicholas Rui Wen Jong, Joan Jing Yi Weng, Xuehuang Jia, Liming Salojärvi, Jarkko Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
description |
Sapindus is an important forest tree genus with utilization in biodiesel, biomedicine, and it harbors great potential for biochemical engineering applications. For advanced breeding of Sapindus, it is necessary to evaluate the genetic diversity and construct a rationally designed core germplasm collection. In this study, the genetic diversity and population structure of Sapindus were conducted with 18 expressed sequence tag-simple sequence repeat (EST-SSR) markers in order to establish a core germplasm collection from 161 Sapindus accessions. The population of Sapindus showed high genetic diversity and significant population structure. Interspecific genetic variation was significantly higher than intraspecific variation in the Sapindus mukorossi, Sapindus delavayi, and combined Sapindus rarak plus Sapindus rarak var. velutinus populations. S. mukorossi had abundant genetic variation and showed a specific pattern of geographical variation, whereas S. delavayi, S. rarak, and S. rarak var. velutinus showed less intraspecific variation. A core germplasm collection was created that contained 40% of genetic variation in the initial population, comprising 53 S. mukorossi and nine S. delavayi lineages, as well as single representatives of S. rarak and S. rarak var. velutinus. These results provide a germplasm basis and theoretical rationale for the efficient management, conservation, and utilization of Sapindus, as well as genetic resources for joint genomics research in the future. |
author2 |
School of Biological Sciences |
author_facet |
School of Biological Sciences Liu, Jiming Gao, Shilun Xu, Yuanyuan Wang, Mianzhi Ngiam, Jia Jun Cho, Nicholas Rui Wen Jong, Joan Jing Yi Weng, Xuehuang Jia, Liming Salojärvi, Jarkko |
format |
Article |
author |
Liu, Jiming Gao, Shilun Xu, Yuanyuan Wang, Mianzhi Ngiam, Jia Jun Cho, Nicholas Rui Wen Jong, Joan Jing Yi Weng, Xuehuang Jia, Liming Salojärvi, Jarkko |
author_sort |
Liu, Jiming |
title |
Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
title_short |
Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
title_full |
Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
title_fullStr |
Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
title_full_unstemmed |
Genetic diversity analysis of sapindus in China and extraction of a core germplasm collection using EST-SSR markers |
title_sort |
genetic diversity analysis of sapindus in china and extraction of a core germplasm collection using est-ssr markers |
publishDate |
2022 |
url |
https://hdl.handle.net/10356/161052 |
_version_ |
1759857357321404416 |