The lupus autoantigen La/Ssb is an Xist-binding protein involved in Xist folding and cloud formation

• Main Authors: Ha, Norbert, Ding, Nan, Hong, Ru, Liu, Rubing, Roca, Xavier, Luo, Yingyuan, Duan, Xiaowei, Wang, Xiao, Ni, Peiling, Wu, Haiyang, Zhang, Li-Feng, Chen, Lingyi
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences; Binding Protein; Animal Cell
• Online Access: https://hdl.handle.net/10356/161273
• Institution: Nanyang Technological University

Using the programmable RNA-sequence binding domain of the Pumilio protein, we FLAG-tagged Xist (inactivated X chromosome specific transcript) in live mouse cells. Affinity pulldown coupled to mass spectrometry was employed to identify a list of 138 candidate Xist-binding proteins, from which, Ssb (a...