Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells

Bisphenol analogues (BPs) are widely used in plastics, food packaging and other commercial products as non safer alternative of BPA. As emerging environmental contaminants, BPs have received considerable attention for their adverse effects on human health. However, their effects on liver metabolisms...

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Main Authors: Jia, Shenglan, Li, Caixia, Fang, Mingliang, Marques Dos Santos, Mauricius, Snyder, Shane Allen
Other Authors: Nanyang Environment and Water Research Institute
Format: Article
Language:English
Published: 2022
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Online Access:https://hdl.handle.net/10356/161822
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1618222022-09-21T01:39:48Z Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells Jia, Shenglan Li, Caixia Fang, Mingliang Marques Dos Santos, Mauricius Snyder, Shane Allen Nanyang Environment and Water Research Institute Engineering::Environmental engineering Bisphenol Analogues Metabolomics Bisphenol analogues (BPs) are widely used in plastics, food packaging and other commercial products as non safer alternative of BPA. As emerging environmental contaminants, BPs have received considerable attention for their adverse effects on human health. However, their effects on liver metabolisms are only marginally understood. In this study, high-resolution mass spectrometry-based global metabolomics and extracellular flux (XF) analysis were applied to characterize the cellular metabolome alterations and reveal the possible mechanisms of the metabolic disorders associated with BPs-induced toxicity in HepG2 cells. BPE, BPB and BPAP with similar chemical structures were selected to compare their interference with different metabolic pathways. A total of 61 key metabolite profiles were significantly altered after exposure to the three BPs. Overall, BPs altered metabolites which are associated with energy metabolism, oxidative stress, cell proliferation and nucleotides synthesis. The primary dysregulated pathways included energy and nucleotides synthesis related Purine and Glycolysis/Gluconeogenesis metabolism. In addition, attenuated mitochondrial function and enhanced glycolysis were found under BPB and BPAP treatment. While attenuated glycolysis was observed under BPE treatment. These findings may provide potential biomarkers indicating the cytotoxicity of BPs and prompt a deeper understanding of the intramolecular metabolic processes induced by BPs exposure. Economic Development Board (EDB) This work is funded by the Economic Development Board - Singapore (S16-1309-NRF-Water-RCFS). 2022-09-21T01:39:48Z 2022-09-21T01:39:48Z 2022 Journal Article Jia, S., Li, C., Fang, M., Marques Dos Santos, M. & Snyder, S. A. (2022). Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells. Chemosphere, 297, 134088-. https://dx.doi.org/10.1016/j.chemosphere.2022.134088 0045-6535 https://hdl.handle.net/10356/161822 10.1016/j.chemosphere.2022.134088 35216976 2-s2.0-85125625706 297 134088 en S16-1309-NRF-Water-RCFS Chemosphere © 2022 Elsevier Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Bisphenol Analogues
Metabolomics
spellingShingle Engineering::Environmental engineering
Bisphenol Analogues
Metabolomics
Jia, Shenglan
Li, Caixia
Fang, Mingliang
Marques Dos Santos, Mauricius
Snyder, Shane Allen
Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
description Bisphenol analogues (BPs) are widely used in plastics, food packaging and other commercial products as non safer alternative of BPA. As emerging environmental contaminants, BPs have received considerable attention for their adverse effects on human health. However, their effects on liver metabolisms are only marginally understood. In this study, high-resolution mass spectrometry-based global metabolomics and extracellular flux (XF) analysis were applied to characterize the cellular metabolome alterations and reveal the possible mechanisms of the metabolic disorders associated with BPs-induced toxicity in HepG2 cells. BPE, BPB and BPAP with similar chemical structures were selected to compare their interference with different metabolic pathways. A total of 61 key metabolite profiles were significantly altered after exposure to the three BPs. Overall, BPs altered metabolites which are associated with energy metabolism, oxidative stress, cell proliferation and nucleotides synthesis. The primary dysregulated pathways included energy and nucleotides synthesis related Purine and Glycolysis/Gluconeogenesis metabolism. In addition, attenuated mitochondrial function and enhanced glycolysis were found under BPB and BPAP treatment. While attenuated glycolysis was observed under BPE treatment. These findings may provide potential biomarkers indicating the cytotoxicity of BPs and prompt a deeper understanding of the intramolecular metabolic processes induced by BPs exposure.
author2 Nanyang Environment and Water Research Institute
author_facet Nanyang Environment and Water Research Institute
Jia, Shenglan
Li, Caixia
Fang, Mingliang
Marques Dos Santos, Mauricius
Snyder, Shane Allen
format Article
author Jia, Shenglan
Li, Caixia
Fang, Mingliang
Marques Dos Santos, Mauricius
Snyder, Shane Allen
author_sort Jia, Shenglan
title Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
title_short Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
title_full Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
title_fullStr Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
title_full_unstemmed Non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
title_sort non-targeted metabolomics revealing the effects of bisphenol analogues on human liver cancer cells
publishDate 2022
url https://hdl.handle.net/10356/161822
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