In vivo liquid-liquid phase separation protects amyloidogenic and aggregation-prone peptides during overexpression in Escherichia coli

• Main Authors: Gabryelczyk, Bartosz, Alag, Reema, Philips, Margaret, Low, Kimberly, Venkatraman, Anandalakshmi, Kannaian, Bhuvaneswari, Shi, Xiangyan, Linder, Markus, Pervushin, Konstantin, Miserez, Ali
• 其他作者: School of Materials Science and Engineering
• 格式: Article
• 語言: English
• 出版: 2022
• 主題: Engineering::Materials; Science::Biological sciences; Amyloids; Membraneless Organelles
• 在線閱讀: https://hdl.handle.net/10356/162100
• 機構: Nanyang Technological University

Studying pathogenic effects of amyloids requires homogeneous amyloidogenic peptide samples. Recombinant production of these peptides is challenging due to their susceptibility to aggregation and chemical modifications. Thus, chemical synthesis is primarily used to produce amyloidogenic peptides suit...