An activatable polymeric nanoprobe for fluorescence and photoacoustic imaging of tumor-associated neutrophils in cancer immunotherapy

• Main Authors: Zhang, Yan, He, Shasha, Xu, Cheng, Jiang, Yue, Miao, Qingqing, Pu, Kanyi
• 其他作者: School of Chemical and Biomedical Engineering
• 格式: Article
• 語言: English
• 出版: 2022
• 主題: Engineering::Bioengineering; Science::Medicine; Activatable Probe; Cancer Immunotherapy
• 在線閱讀: https://hdl.handle.net/10356/162212
• 機構: Nanyang Technological University

Imaging to evaluate tumor-associated neutrophils (TANs) is imperative for cancer immunotherapy but remains challenging. We herein report an activatable semiconducting polymer nanoprobe (SPCy) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of neutrophil elastase (NE), a biomarker of TANs. SPCy comprises a semiconducting polymer conjugated with a hemicyanine (hemi-Cy) dye caged by a NE-cleavable peptide as the side chain. After systemic administration, SPCy passively targets the tumor and reacts with NE to "uncage" the hemi-Cy, leading to enhanced NIRF and PA signals of the hemi-Cy but unchanged signals of the SP. Such NE-activated ratiometric NIRF and enhanced PA signals of SPCy correlate with the intratumoral population of TANs. Thus, this study not only presents the first TAN-specific PA probe, but also provides a general molecular design strategy for PA imaging of other immune-related biomarkers to facilitate screening of cancer immunotherapeutics.