Characterisation and functional study of activation function 1 (AF-1) of progesterone receptor in mouse model

• Main Author: Lee, Shi Hao
• Other Authors: Valerie Lin
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2022
• Subjects: Science::Biological sciences::Molecular biology
• Online Access: https://hdl.handle.net/10356/162450
• Institution: Nanyang Technological University

Progesterone receptor (PR) is a master regulator in many female reproductive functions. In the uterus, PR regulates its function through antagonistic as well as synergistic interplays with estrogen receptors. PR also regulates mammary gland development especially during pregnancy and puberty. While PR action is primarily mediate through activation functions 1 and 2 (AF-1 and AF-2), their physiological significance is unknown. We report the first study of AF1 function in mice. Mutant PR AF-1 mice displayed uterine physiological defects, infertility coupled with impaired implantation and decidualisation. Mutant mice also displayed severe mammary gland development during pregnancy. Here, we seek to understand and unpack the physiological significance of AF-1 in PR activity in mice.