HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.

Hepatocellular carcinoma (HCC) is frequently found in patients with chronic infection of either hepatitis B- or C- virus (HBV or HCV). However, the precise mechanism of how these viral proteins are involved in hepatocarcinogenesis remains unknown. The nuclear receptor co-repressor (N-CoR) and other...

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Main Author: Haris.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2009
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Online Access:http://hdl.handle.net/10356/16348
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-163482023-02-28T18:02:11Z HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation. Haris. School of Biological Sciences Oncology Research Institute National Univeristy of Singapore Matiullah Khan DRNTU::Science::Biological sciences::Molecular biology Hepatocellular carcinoma (HCC) is frequently found in patients with chronic infection of either hepatitis B- or C- virus (HBV or HCV). However, the precise mechanism of how these viral proteins are involved in hepatocarcinogenesis remains unknown. The nuclear receptor co-repressor (N-CoR) and other promyelocytic leukemia oncogenic domain (POD) components have been shown to involve in transcriptional repression function. In this preliminary observation, we detected significant degradation of N-CoR in HBV-containing human hepatoma cell lines. Furthermore, our western blot analyses and localization experiments in 293T (human embryonic kidney) cells demonstrated that hepatitis viral oncogenic proteins, HBV X protein (HBx) and HCV core, disrupted N-CoR-mediated transcriptional repression function by triggering cytosolic localization of N-CoR and its subsequent degradation. Disruption of this biological function suggests the capability of HBV and HCV to overcome the nuclear defense mechanisms exerted by POD which commonly showed structural disintegration in several viral infections. In addition, up-regulation of genes related to cell proliferation in HCC might also be associated with the loss of N-CoR-mediated transcriptional repression activity upon chronic infection by HBV or HCV. Hence, the novel molecular mechanism postulated here can be exploited for therapeutic approaches against HBV and HCV infections and more importantly, in the treatment of HCC. Bachelor of Science in Biological Sciences 2009-05-25T07:02:10Z 2009-05-25T07:02:10Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16348 en Nanyang Technological University 27 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Haris.
HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
description Hepatocellular carcinoma (HCC) is frequently found in patients with chronic infection of either hepatitis B- or C- virus (HBV or HCV). However, the precise mechanism of how these viral proteins are involved in hepatocarcinogenesis remains unknown. The nuclear receptor co-repressor (N-CoR) and other promyelocytic leukemia oncogenic domain (POD) components have been shown to involve in transcriptional repression function. In this preliminary observation, we detected significant degradation of N-CoR in HBV-containing human hepatoma cell lines. Furthermore, our western blot analyses and localization experiments in 293T (human embryonic kidney) cells demonstrated that hepatitis viral oncogenic proteins, HBV X protein (HBx) and HCV core, disrupted N-CoR-mediated transcriptional repression function by triggering cytosolic localization of N-CoR and its subsequent degradation. Disruption of this biological function suggests the capability of HBV and HCV to overcome the nuclear defense mechanisms exerted by POD which commonly showed structural disintegration in several viral infections. In addition, up-regulation of genes related to cell proliferation in HCC might also be associated with the loss of N-CoR-mediated transcriptional repression activity upon chronic infection by HBV or HCV. Hence, the novel molecular mechanism postulated here can be exploited for therapeutic approaches against HBV and HCV infections and more importantly, in the treatment of HCC.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Haris.
format Final Year Project
author Haris.
author_sort Haris.
title HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
title_short HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
title_full HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
title_fullStr HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
title_full_unstemmed HBx and HCV core, viral proteins associated with hepatocarcinoma, promote N-CoR degradation.
title_sort hbx and hcv core, viral proteins associated with hepatocarcinoma, promote n-cor degradation.
publishDate 2009
url http://hdl.handle.net/10356/16348
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