The cellular characterization of SARS-CoV-2 spike protein in virus-infected cells using the receptor binding domain binding specific human monoclonal antibodies

• Main Authors: Chan, Conrad En-Zuo, Ng, Ching-Ging, Lim, Angeline Pei-Chew, Seah, Shirley Lay-Kheng, Chye, De-Hoe, Wong, Steven Ka-Khuen, Lim, Jie-Hui, Lim, Vanessa Zi-Yun, Lai, Soak-Kuan, Wong, Pui-San, Leong, Kok-Mun, Liu, Yi-Chun, Sugrue, Richard J., Tan, Boon Huan
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Medicine; S Protein; Receptor Binding Domain
• Online Access: https://hdl.handle.net/10356/163880
• Institution: Nanyang Technological University

A human monoclonal antibody panel (PD4, PD5, PD7, SC23, and SC29) was isolated from the B cells of convalescent patients and used to examine the S protein in SARS-CoV-2-infected cells. While all five antibodies bound conformational-specific epitopes within SARS-CoV-2 spike (S) protein, only PD5, PD7...