Expression optimisation of antimicrobial fusion protein variants

• Main Author: Wong, Long Hui.
• Other Authors: Susanna Leong Su Jan
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/16393
• Institution: Nanyang Technological University

This work reports the recombinant expression of human beta-defensin 25 fusion proteins in E. coli and various transformation experiments carried out using recombinant plasmids. HBD-25 is found to be highly expressed in the human epididymis. Like other human defensins, its ability to act as a host...