MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greate...
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sg-ntu-dr.10356-1640522023-02-28T17:13:06Z MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P. Han, Jongyoon Preiser, Peter Rainer School of Biological Sciences BioSystems and Micromechanics (BioSyM) interdisciplinary research group (IRG), Singapore-MIT Alliance for Research and Technology (SMART) Anti-Microbial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART) Science::Biological sciences High-Throughput Screening Comet Assay Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020). National Research Foundation (NRF) Published version This work was supported by the National Research Foundation, Prime Minister’s Office, Singapore, through the Singapore-MIT Alliance for Research and Technology (SMART) BioSystems and Micromechanics (BioSym) Interdisciplinary Research Group (IRG) (to A.X. and J.H.) and Antimicrobial Resistance IRG (to A.X., J.H., and P.R.P.); by the Bill and Melinda Gates Foundation (Global Health grant number OPP1040463 to A.X. and P.R.P.); S.K. was supported by the National Institute of Environmental Health Sciences Superfund Basic Research Program (NIH P42 ES027707) to promote research translation. 2023-01-03T08:07:55Z 2023-01-03T08:07:55Z 2021 Journal Article Xiong, A., Kaushal, S., Tay, I. J., Engelward, B. P., Han, J. & Preiser, P. R. (2021). MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum. STAR Protocols, 2(3), 100797-. https://dx.doi.org/10.1016/j.xpro.2021.100797 2666-1667 https://hdl.handle.net/10356/164052 10.1016/j.xpro.2021.100797 34527954 2-s2.0-85122802034 3 2 100797 en STAR Protocols © 2021 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
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Science::Biological sciences High-Throughput Screening Comet Assay Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P. Han, Jongyoon Preiser, Peter Rainer MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| description |
Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020). |
| author2 |
School of Biological Sciences |
| author_facet |
School of Biological Sciences Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P. Han, Jongyoon Preiser, Peter Rainer |
| format |
Article |
| author |
Xiong, Aoli Kaushal, Simran Tay, Ian Junjie Engelward, Bevin P. Han, Jongyoon Preiser, Peter Rainer |
| author_sort |
Xiong, Aoli |
| title |
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_short |
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full |
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_fullStr |
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full_unstemmed |
MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_sort |
malariacometchip for high-throughput quantification of dna damage in plasmodium falciparum |
| publishDate |
2023 |
| url |
https://hdl.handle.net/10356/164052 |
| _version_ |
1759855430219071488 |