Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells
O'nyongnyong virus (ONNV) is a re-emerging alphavirus previously known to be transmitted by main malaria vectors, thus suggesting the possibility of coinfections with arboviruses in co-endemic areas. However, the pathological outcomes of such infections remain unknown. Using murine coinfection...
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sg-ntu-dr.10356-1642142023-02-28T17:12:45Z Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells Torres-Ruesta, Anthony Teo, Teck-Hui Chan, Yi-Hao Siti Naqiah Amrun Yeo, Nicholas Kim-Wah Lee, Cheryl Yi-Pin Nguee, Samantha Yee-Teng Tay, Matthew Zirui Nosten, Francois Fong, Siew-Wai Lum, Fok-Moon Carissimo, Guillaume Renia, Laurent Ng, Lisa F. P. Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences A*STAR Infectious Diseases Labs Singapore Immunology Network, A*STAR Science::Medicine Alphavirus Infection Malaria O'nyongnyong virus (ONNV) is a re-emerging alphavirus previously known to be transmitted by main malaria vectors, thus suggesting the possibility of coinfections with arboviruses in co-endemic areas. However, the pathological outcomes of such infections remain unknown. Using murine coinfection models, we demonstrated that a preexisting blood-stage Plasmodium infection suppresses ONNV-induced pathologies. We further showed that suppression of viremia and virus dissemination are dependent on Plasmodium-induced IFNγ and are associated with reduced infection of CD45- cells at the site of virus inoculation. We further proved that treatment with IFNγ or plasma samples from Plasmodium vivax-infected patients containing IFNγ are able to restrict ONNV infection in human fibroblast, synoviocyte, skeletal muscle, and endothelial cell lines. Mechanistically, the role of IFNγ in restricting ONNV infection was confirmed in in vitro infection assays through the generation of an IFNγ receptor 1 α chain (IFNγR1)-deficient cell line. Agency for Science, Technology and Research (A*STAR) Published version The study was supported by a core research grant provided to A*STAR Infectious Diseases Labs and Singapore Immunology Network by the Biomedical Research Council (BMRC) from the Agency for Science, Technology and Research (A*STAR). A Torres-Ruesta is supported by the A*STAR Singapore International Graduate Award (SINGA) scholarship. Flow cytometry platform is supported by the Health and Biomedical Sciences (HBMS) Open Fund Shared Infrastructure Support Grant under the Immunomonitoring Service Platform project (NRF2017_SISFP09). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. 2023-01-10T01:14:06Z 2023-01-10T01:14:06Z 2022 Journal Article Torres-Ruesta, A., Teo, T., Chan, Y., Siti Naqiah Amrun, Yeo, N. K., Lee, C. Y., Nguee, S. Y., Tay, M. Z., Nosten, F., Fong, S., Lum, F., Carissimo, G., Renia, L. & Ng, L. F. P. (2022). Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells. Life Science Alliance, 5(4), e202101272-. https://dx.doi.org/10.26508/lsa.202101272 2575-1077 https://hdl.handle.net/10356/164214 10.26508/lsa.202101272 35039441 2-s2.0-85123568157 4 5 e202101272 en NRF2017_SISFP09 Life Science Alliance © 2022 Torres-Ruesta et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). application/pdf |
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Science::Medicine Alphavirus Infection Malaria Torres-Ruesta, Anthony Teo, Teck-Hui Chan, Yi-Hao Siti Naqiah Amrun Yeo, Nicholas Kim-Wah Lee, Cheryl Yi-Pin Nguee, Samantha Yee-Teng Tay, Matthew Zirui Nosten, Francois Fong, Siew-Wai Lum, Fok-Moon Carissimo, Guillaume Renia, Laurent Ng, Lisa F. P. Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
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O'nyongnyong virus (ONNV) is a re-emerging alphavirus previously known to be transmitted by main malaria vectors, thus suggesting the possibility of coinfections with arboviruses in co-endemic areas. However, the pathological outcomes of such infections remain unknown. Using murine coinfection models, we demonstrated that a preexisting blood-stage Plasmodium infection suppresses ONNV-induced pathologies. We further showed that suppression of viremia and virus dissemination are dependent on Plasmodium-induced IFNγ and are associated with reduced infection of CD45- cells at the site of virus inoculation. We further proved that treatment with IFNγ or plasma samples from Plasmodium vivax-infected patients containing IFNγ are able to restrict ONNV infection in human fibroblast, synoviocyte, skeletal muscle, and endothelial cell lines. Mechanistically, the role of IFNγ in restricting ONNV infection was confirmed in in vitro infection assays through the generation of an IFNγ receptor 1 α chain (IFNγR1)-deficient cell line. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Torres-Ruesta, Anthony Teo, Teck-Hui Chan, Yi-Hao Siti Naqiah Amrun Yeo, Nicholas Kim-Wah Lee, Cheryl Yi-Pin Nguee, Samantha Yee-Teng Tay, Matthew Zirui Nosten, Francois Fong, Siew-Wai Lum, Fok-Moon Carissimo, Guillaume Renia, Laurent Ng, Lisa F. P. |
format |
Article |
author |
Torres-Ruesta, Anthony Teo, Teck-Hui Chan, Yi-Hao Siti Naqiah Amrun Yeo, Nicholas Kim-Wah Lee, Cheryl Yi-Pin Nguee, Samantha Yee-Teng Tay, Matthew Zirui Nosten, Francois Fong, Siew-Wai Lum, Fok-Moon Carissimo, Guillaume Renia, Laurent Ng, Lisa F. P. |
author_sort |
Torres-Ruesta, Anthony |
title |
Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
title_short |
Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
title_full |
Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
title_fullStr |
Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
title_full_unstemmed |
Malaria abrogates O'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
title_sort |
malaria abrogates o'nyong-nyong virus pathologies by restricting virus infection in nonimmune cells |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/164214 |
_version_ |
1759854778491338752 |