Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants

There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccin...

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Bibliographic Details
Main Authors: Lim, Sing Mei, Cheng, Hoi Lok, Jia, Huan, Kongsuphol, Patthara, Bhuvaneshwari D/O Shunmuganathan, Chen, Ming Wei, Ng, Say Yong, Gao, Xiaohong, Turaga, Shuvan Prashant, Heussler, Sascha P., Somani, Jyoti, Sengupta, Sharmila, Tay, Dousabel M. Y., McBee, Megan E., Young, Barnaby Edward, MacAry, Paul A., Sikes, Hadley D., Preiser, Peter Rainer
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164355
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Institution: Nanyang Technological University
Language: English
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Summary:There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.