Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants
There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccin...
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sg-ntu-dr.10356-1643552023-02-28T17:12:52Z Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants Lim, Sing Mei Cheng, Hoi Lok Jia, Huan Kongsuphol, Patthara Bhuvaneshwari D/O Shunmuganathan Chen, Ming Wei Ng, Say Yong Gao, Xiaohong Turaga, Shuvan Prashant Heussler, Sascha P. Somani, Jyoti Sengupta, Sharmila Tay, Dousabel M. Y. McBee, Megan E. Young, Barnaby Edward MacAry, Paul A. Sikes, Hadley D. Preiser, Peter Rainer School of Biological Sciences Lee Kong Chian School of Medicine (LKCMedicine) Singapore-MIT Alliance in Research and Technology (SMART) National Centre for Infectious Diseases Tan Tock Seng Hospital Science::Medicine Cellulose Pulldown Assay COVID19 There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population. Ministry of Health (MOH) National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This study is supported by National Health Innovation Singapore (NHIC) grant # NHIC-COVID19-2005004, National Research Foundation via CREATE Share grant #R571-002-021-592 and the Anti-microbial Resistance Interdisciplinary Research Group (AMR-IRG) of Singapore-MIT Alliance in Research and Technology (SMART). All samples acquired from National Centre for Infectious Diseases (NCID) were supported by Singapore Ministry of Health's National Medical Research Council COVID-19 Research Fund: COVID19RF-0008. 2023-01-17T05:42:39Z 2023-01-17T05:42:39Z 2022 Journal Article Lim, S. M., Cheng, H. L., Jia, H., Kongsuphol, P., Bhuvaneshwari D/O Shunmuganathan, Chen, M. W., Ng, S. Y., Gao, X., Turaga, S. P., Heussler, S. P., Somani, J., Sengupta, S., Tay, D. M. Y., McBee, M. E., Young, B. E., MacAry, P. A., Sikes, H. D. & Preiser, P. R. (2022). Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants. Bioengineering and Translational Medicine, 7(2), e10293-. https://dx.doi.org/10.1002/btm2.10293 2380-6761 https://hdl.handle.net/10356/164355 10.1002/btm2.10293 35600666 2-s2.0-85124629370 2 7 e10293 en NHIC-COVID19-2005004 #R571-002-021-592 COVID19RF-0008 Bioengineering and Translational Medicine © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. application/pdf |
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Science::Medicine Cellulose Pulldown Assay COVID19 Lim, Sing Mei Cheng, Hoi Lok Jia, Huan Kongsuphol, Patthara Bhuvaneshwari D/O Shunmuganathan Chen, Ming Wei Ng, Say Yong Gao, Xiaohong Turaga, Shuvan Prashant Heussler, Sascha P. Somani, Jyoti Sengupta, Sharmila Tay, Dousabel M. Y. McBee, Megan E. Young, Barnaby Edward MacAry, Paul A. Sikes, Hadley D. Preiser, Peter Rainer Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
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There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population. |
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School of Biological Sciences |
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School of Biological Sciences Lim, Sing Mei Cheng, Hoi Lok Jia, Huan Kongsuphol, Patthara Bhuvaneshwari D/O Shunmuganathan Chen, Ming Wei Ng, Say Yong Gao, Xiaohong Turaga, Shuvan Prashant Heussler, Sascha P. Somani, Jyoti Sengupta, Sharmila Tay, Dousabel M. Y. McBee, Megan E. Young, Barnaby Edward MacAry, Paul A. Sikes, Hadley D. Preiser, Peter Rainer |
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Article |
author |
Lim, Sing Mei Cheng, Hoi Lok Jia, Huan Kongsuphol, Patthara Bhuvaneshwari D/O Shunmuganathan Chen, Ming Wei Ng, Say Yong Gao, Xiaohong Turaga, Shuvan Prashant Heussler, Sascha P. Somani, Jyoti Sengupta, Sharmila Tay, Dousabel M. Y. McBee, Megan E. Young, Barnaby Edward MacAry, Paul A. Sikes, Hadley D. Preiser, Peter Rainer |
author_sort |
Lim, Sing Mei |
title |
Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
title_short |
Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
title_full |
Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
title_fullStr |
Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
title_full_unstemmed |
Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants |
title_sort |
finger stick blood test to assess postvaccination sars-cov-2 neutralizing antibody response against variants |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/164355 |
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1759853717162557440 |