Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals

This dissertation describes advanced crystallization and precipitation strategies to improve process efficiency and product quality of biopharmaceuticals using bioactive glycopeptide vancomycin hydrochloride (Van) and protein lysozyme (LYZ) as the model biopharmaceuticals. The first study focuses on...

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Main Author: Pu, Siyu
Other Authors: Kunn Hadinoto Ong
Format: Thesis-Doctor of Philosophy
Language:English
Published: Nanyang Technological University 2023
Subjects:
Online Access:https://hdl.handle.net/10356/164549
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1645492023-03-06T07:30:04Z Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals Pu, Siyu Kunn Hadinoto Ong School of Chemical and Biomedical Engineering KunnOng@ntu.edu.sg Engineering::Chemical engineering This dissertation describes advanced crystallization and precipitation strategies to improve process efficiency and product quality of biopharmaceuticals using bioactive glycopeptide vancomycin hydrochloride (Van) and protein lysozyme (LYZ) as the model biopharmaceuticals. The first study focuses on the phase behavior of salting-out room-temperature crystallization of Van to determine crystallization conditions (i.e., pH, salt and peptide’s concentrations, incubation time) to avoid needle-shaped crystal formation. The second study compared the antisolvent (with organic solvent) and salting-out methods in their precipitation efficiency and product qualities of Van. In the third study, we evaluated for the first time the use of continuous slug flow crystallizer (CSFC) in bulk seeded protein crystallization, using lysozyme (LYZ) as the model protein. Lastly, in the fourth study, we developed a segmented slug flow crystallizer (SFC) of LYZ operated at a short residence time to improve the width of the crystal size distribution (CSD) and CSD’s reproducibility. Doctor of Philosophy 2023-02-01T08:43:30Z 2023-02-01T08:43:30Z 2022 Thesis-Doctor of Philosophy Pu, S. (2022). Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/164549 https://hdl.handle.net/10356/164549 10.32657/10356/164549 en 10.1016/j.ces.2022.118057 10.1016/j.cherd.2021.05.011 10.1016/j.cherd.2020.05.004 10.1016/j.ces.2020.116181 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
spellingShingle Engineering::Chemical engineering
Pu, Siyu
Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
description This dissertation describes advanced crystallization and precipitation strategies to improve process efficiency and product quality of biopharmaceuticals using bioactive glycopeptide vancomycin hydrochloride (Van) and protein lysozyme (LYZ) as the model biopharmaceuticals. The first study focuses on the phase behavior of salting-out room-temperature crystallization of Van to determine crystallization conditions (i.e., pH, salt and peptide’s concentrations, incubation time) to avoid needle-shaped crystal formation. The second study compared the antisolvent (with organic solvent) and salting-out methods in their precipitation efficiency and product qualities of Van. In the third study, we evaluated for the first time the use of continuous slug flow crystallizer (CSFC) in bulk seeded protein crystallization, using lysozyme (LYZ) as the model protein. Lastly, in the fourth study, we developed a segmented slug flow crystallizer (SFC) of LYZ operated at a short residence time to improve the width of the crystal size distribution (CSD) and CSD’s reproducibility.
author2 Kunn Hadinoto Ong
author_facet Kunn Hadinoto Ong
Pu, Siyu
format Thesis-Doctor of Philosophy
author Pu, Siyu
author_sort Pu, Siyu
title Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
title_short Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
title_full Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
title_fullStr Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
title_full_unstemmed Advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
title_sort advanced strategies to enhance the performances of crystallization and precipitation of biopharmaceuticals
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/164549
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