Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes

The present study investigated the molecular phylogeny, antimicrobial and cytotoxic activities of fungal endophytes obtained from the A*STAR Natural Organism Library (NOL) and previously isolated from Sungei Buloh Wetland Reserve, Singapore. Phylogenetic analysis based on ITS2 gene suggests that the...

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Main Authors: Munusamy, Madhaiyan, Ching, Kuan Chieh, Yang, Lay Kien, Crasta, Sharon, Gakuubi, Martin Muthee, Chee, Zhao Yan, Wibowo, Mario, Leong, Chung Yan, Kanagasundaram, Yoganathan, Ng, Siew Bee
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164777
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1647772023-02-28T17:14:05Z Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes Munusamy, Madhaiyan Ching, Kuan Chieh Yang, Lay Kien Crasta, Sharon Gakuubi, Martin Muthee Chee, Zhao Yan Wibowo, Mario Leong, Chung Yan Kanagasundaram, Yoganathan Ng, Siew Bee School of Biological Sciences Science::Biological sciences Chemical Elicitors Pestahivin Analogues The present study investigated the molecular phylogeny, antimicrobial and cytotoxic activities of fungal endophytes obtained from the A*STAR Natural Organism Library (NOL) and previously isolated from Sungei Buloh Wetland Reserve, Singapore. Phylogenetic analysis based on ITS2 gene suggests that these isolates belong to 46 morphotypes and are affiliated to 23 different taxa in 17 genera of the Ascomycota phylum. Colletotrichum was the most dominant fungal genus accounting for 37% of all the isolates, followed by Diaporthe (13%), Phyllosticta (10.9%) and Diplodia (8.7%). Chemical elicitation using 5-azacytidine, a DNA methyltransferase inhibitor and suberoylanilide hydroxamic acid, a histone deacetylase inhibitor resulted in an increase in the number of active strains. Bioassay-guided isolation and structural elucidation yielded pestahivin and two new analogues from Bartalinia sp. F9447. Pestahivin and its related analogues did not exhibit antibacterial activity against Staphylococcus aureus but displayed strong antifungal activities against Candida albicans and Aspergillus brasiliensis, with IC50 values ranging from 0.46 ± 0.06 to 144 ± 18 µM. Pestahivin and its two analogues furthermore exhibited cytotoxic activity against A549 and MIA PACA-2 cancer cell lines with IC50 values in the range of 0.65 ± 0.12 to 42 ± 5.2 µM. The finding from this study reinforces that chemical epigenetic induction is a promising approach for the discovery of bioactive fungal secondary metabolites encoded by cryptic gene clusters. Agency for Science, Technology and Research (A*STAR) Published version We acknowledge the funding support from Agency for Science, Technology and Research (A*STAR), Singapore, through a Singapore Integrative Biosystems and Engineering Research (SIBER) Grant (#21719). 2023-02-14T02:36:32Z 2023-02-14T02:36:32Z 2022 Journal Article Munusamy, M., Ching, K. C., Yang, L. K., Crasta, S., Gakuubi, M. M., Chee, Z. Y., Wibowo, M., Leong, C. Y., Kanagasundaram, Y. & Ng, S. B. (2022). Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes. Frontiers in Chemistry, 10, 1024854-. https://dx.doi.org/10.3389/fchem.2022.1024854 2296-2646 https://hdl.handle.net/10356/164777 10.3389/fchem.2022.1024854 36505735 2-s2.0-85143404493 10 1024854 en #21719 Frontiers in Chemistry © 2022 Munusamy, Ching, Yang, Crasta, Gakuubi, Chee, Wibowo, Leong, Kanagasundaram and Ng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Chemical Elicitors
Pestahivin Analogues
spellingShingle Science::Biological sciences
Chemical Elicitors
Pestahivin Analogues
Munusamy, Madhaiyan
Ching, Kuan Chieh
Yang, Lay Kien
Crasta, Sharon
Gakuubi, Martin Muthee
Chee, Zhao Yan
Wibowo, Mario
Leong, Chung Yan
Kanagasundaram, Yoganathan
Ng, Siew Bee
Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
description The present study investigated the molecular phylogeny, antimicrobial and cytotoxic activities of fungal endophytes obtained from the A*STAR Natural Organism Library (NOL) and previously isolated from Sungei Buloh Wetland Reserve, Singapore. Phylogenetic analysis based on ITS2 gene suggests that these isolates belong to 46 morphotypes and are affiliated to 23 different taxa in 17 genera of the Ascomycota phylum. Colletotrichum was the most dominant fungal genus accounting for 37% of all the isolates, followed by Diaporthe (13%), Phyllosticta (10.9%) and Diplodia (8.7%). Chemical elicitation using 5-azacytidine, a DNA methyltransferase inhibitor and suberoylanilide hydroxamic acid, a histone deacetylase inhibitor resulted in an increase in the number of active strains. Bioassay-guided isolation and structural elucidation yielded pestahivin and two new analogues from Bartalinia sp. F9447. Pestahivin and its related analogues did not exhibit antibacterial activity against Staphylococcus aureus but displayed strong antifungal activities against Candida albicans and Aspergillus brasiliensis, with IC50 values ranging from 0.46 ± 0.06 to 144 ± 18 µM. Pestahivin and its two analogues furthermore exhibited cytotoxic activity against A549 and MIA PACA-2 cancer cell lines with IC50 values in the range of 0.65 ± 0.12 to 42 ± 5.2 µM. The finding from this study reinforces that chemical epigenetic induction is a promising approach for the discovery of bioactive fungal secondary metabolites encoded by cryptic gene clusters.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Munusamy, Madhaiyan
Ching, Kuan Chieh
Yang, Lay Kien
Crasta, Sharon
Gakuubi, Martin Muthee
Chee, Zhao Yan
Wibowo, Mario
Leong, Chung Yan
Kanagasundaram, Yoganathan
Ng, Siew Bee
format Article
author Munusamy, Madhaiyan
Ching, Kuan Chieh
Yang, Lay Kien
Crasta, Sharon
Gakuubi, Martin Muthee
Chee, Zhao Yan
Wibowo, Mario
Leong, Chung Yan
Kanagasundaram, Yoganathan
Ng, Siew Bee
author_sort Munusamy, Madhaiyan
title Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
title_short Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
title_full Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
title_fullStr Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
title_full_unstemmed Chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
title_sort chemical elicitation as an avenue for discovery of bioactive compounds from fungal endophytes
publishDate 2023
url https://hdl.handle.net/10356/164777
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