Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1

Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1

Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecul...

Full description

Saved in:
Bibliographic Details
Main Authors: Zhang, Kuo, Law, Michelle Cheok Yien, Nguyen, Trinh Mai, Tan, Yaw Bia, Wirawan, Melissa, Law, Yee-Song, Jeong, Lak Shin, Luo, Dahai
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2023
Subjects:
Science::Medicine
Science::Biological sciences
mRNA
Chikungunya Virus
Online Access:https://hdl.handle.net/10356/164882
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
Description
Summary:Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecular basis of the capping process remains unclear. We determine high-resolution cryoelectron microscopy (cryo-EM) structures of Chikungunya virus nsP1 in complex with m7GTP/SAH, covalently attached m7GMP, and Cap-0 viral RNA. These structures reveal details of viral-RNA-capping reactions and uncover a sequence-specific virus RNA-recognition pattern that, in turn, regulates viral-RNA-capping efficiency to ensure optimal genome replication and subgenomic RNA transcription. This sequence-specific enzyme-RNA pairing is conserved across all alphaviruses.

Similar Items