Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis

Dental pulp inflammation, or pulpitis, is a common painful dental condition. Connexin 43 (Cx43) is a building block for cellular portals of communication such as hemichannels and gap junctions. These play key roles in coordinating an immune response during pulpitis. It is hypothesized that pathologi...

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Main Author: Lim, Wen Yi
Other Authors: David Lawrence Becker
Format: Thesis-Doctor of Philosophy
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/165279
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1652792023-04-04T02:58:00Z Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis Lim, Wen Yi David Lawrence Becker Lee Kong Chian School of Medicine (LKCMedicine) david.becker@ntu.edu.sg Science::Biological sciences::Human anatomy and physiology Dental pulp inflammation, or pulpitis, is a common painful dental condition. Connexin 43 (Cx43) is a building block for cellular portals of communication such as hemichannels and gap junctions. These play key roles in coordinating an immune response during pulpitis. It is hypothesized that pathologic expression of these conduits causes an excessive inflammatory response leading to increased host collateral destruction. This thesis investigated Cx43 upregulation during pulpitis in extracted human teeth as well as in a rodent pulpitis model. Subsequently, the therapeutic effect of Cx43 downregulation in the treatment of pulpitis was studied. The use of Cx43 antisense-oligodeoxynucleotide as well as the hemichannel blocker Tonabersat (SB-220453) delivered via various scaffolds were investigated. Finally, a pilot was carried out in a large-animal porcine model to examine the long-term effect of Cx43 downregulation as a therapeutic approach, with the long-term goal of providing effective alternatives in the treatment of pulpitis. Doctor of Philosophy 2023-03-22T08:19:03Z 2023-03-22T08:19:03Z 2022 Thesis-Doctor of Philosophy Lim, W. Y. (2022). Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/165279 https://hdl.handle.net/10356/165279 10.32657/10356/165279 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences::Human anatomy and physiology
spellingShingle Science::Biological sciences::Human anatomy and physiology
Lim, Wen Yi
Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
description Dental pulp inflammation, or pulpitis, is a common painful dental condition. Connexin 43 (Cx43) is a building block for cellular portals of communication such as hemichannels and gap junctions. These play key roles in coordinating an immune response during pulpitis. It is hypothesized that pathologic expression of these conduits causes an excessive inflammatory response leading to increased host collateral destruction. This thesis investigated Cx43 upregulation during pulpitis in extracted human teeth as well as in a rodent pulpitis model. Subsequently, the therapeutic effect of Cx43 downregulation in the treatment of pulpitis was studied. The use of Cx43 antisense-oligodeoxynucleotide as well as the hemichannel blocker Tonabersat (SB-220453) delivered via various scaffolds were investigated. Finally, a pilot was carried out in a large-animal porcine model to examine the long-term effect of Cx43 downregulation as a therapeutic approach, with the long-term goal of providing effective alternatives in the treatment of pulpitis.
author2 David Lawrence Becker
author_facet David Lawrence Becker
Lim, Wen Yi
format Thesis-Doctor of Philosophy
author Lim, Wen Yi
author_sort Lim, Wen Yi
title Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
title_short Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
title_full Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
title_fullStr Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
title_full_unstemmed Differential expression of Connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of Connexin 43 downregulation in treatment of pulpitis
title_sort differential expression of connexin 43 during dental pulp inflammation and its role in pulpal healing: a translational study determining effect of connexin 43 downregulation in treatment of pulpitis
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/165279
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