Molecular cloning of novel recombinant plasmids for exogenous expression of PKCε in single living cells
In this study, overexpression was done on the recombinant plasmids of both wild-type PKCε (wt-PKCε) and dominant negative PKCε (dn-PKCε) in PC 12 cells with reporter genes, Enhanced Green Fluorescent Protein at C1 position (pEGFP-C1), pIRES2-EGFP and pIRES2-DsRed. Both fusion and concurrent gene exp...
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Format: | Final Year Project |
Language: | English |
Published: |
2009
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Online Access: | http://hdl.handle.net/10356/16545 |
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Institution: | Nanyang Technological University |
Language: | English |