A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE

• Main Authors: Yap, Wei Sheng, Thibault, Guillaume
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Gene Assembly Method; SCRaMbLE
• Online Access: https://hdl.handle.net/10356/165616
• Institution: Nanyang Technological University

Human proteins expressed in yeast are common to enhance protein production while the expression of functional human pathways remain challenging. Here, we propose a simple and economical high-throughput gene assembly method to create a yeast megaplasmid library from human cDNA to screen for minimal h...