Proteomics of hair: effects of pH on the hair shaft

Evaluating the effect pH has on the hair fibre is important to develop better shampoos that can improve the hair texture and mechanical properties. There has been little investigation into the characterisation of hair shaft proteins and the effect pH exposure has on them. Past research has shown,...

Full description

Saved in:
Bibliographic Details
Main Author: Wu, Alicia Rosabee Yu Ling
Other Authors: Ng Kee Woei
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
Subjects:
Online Access:https://hdl.handle.net/10356/165760
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-165760
record_format dspace
spelling sg-ntu-dr.10356-1657602023-04-15T16:45:51Z Proteomics of hair: effects of pH on the hair shaft Wu, Alicia Rosabee Yu Ling Ng Kee Woei School of Materials Science and Engineering KWNG@ntu.edu.sg Engineering::Materials::Biomaterials Evaluating the effect pH has on the hair fibre is important to develop better shampoos that can improve the hair texture and mechanical properties. There has been little investigation into the characterisation of hair shaft proteins and the effect pH exposure has on them. Past research has shown, however, that there is no advised range of shampoo pH. In this study, hair shaft fragments were immersed in a range of pH solutions (pH 3 to 12) for 15 to 30 minutes to mimic hair wash and several characterisation techniques were used to study the effect it has on the hair shaft and the cuticular and keratin-associated proteins. Scanning electron microscopy (SEM) was used to investigate the effect pH had on the hair morphology. A total of 350 SEM images were taken and analysed against a scale measuring the degree of cuticle lifting and flaking. The results showed that pH greater than 7 presented greater overall surface damage. The samples also underwent protein identification and quantification through liquid chromatography/mass spectrometry and the results showed a greater abundance at acidic pH. This preliminary research can conclude that a lower pH is recommended for shampoos. Bachelor of Engineering (Materials Engineering) 2023-04-10T11:09:14Z 2023-04-10T11:09:14Z 2023 Final Year Project (FYP) Wu, A. R. Y. L. (2023). Proteomics of hair: effects of pH on the hair shaft. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/165760 https://hdl.handle.net/10356/165760 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials::Biomaterials
spellingShingle Engineering::Materials::Biomaterials
Wu, Alicia Rosabee Yu Ling
Proteomics of hair: effects of pH on the hair shaft
description Evaluating the effect pH has on the hair fibre is important to develop better shampoos that can improve the hair texture and mechanical properties. There has been little investigation into the characterisation of hair shaft proteins and the effect pH exposure has on them. Past research has shown, however, that there is no advised range of shampoo pH. In this study, hair shaft fragments were immersed in a range of pH solutions (pH 3 to 12) for 15 to 30 minutes to mimic hair wash and several characterisation techniques were used to study the effect it has on the hair shaft and the cuticular and keratin-associated proteins. Scanning electron microscopy (SEM) was used to investigate the effect pH had on the hair morphology. A total of 350 SEM images were taken and analysed against a scale measuring the degree of cuticle lifting and flaking. The results showed that pH greater than 7 presented greater overall surface damage. The samples also underwent protein identification and quantification through liquid chromatography/mass spectrometry and the results showed a greater abundance at acidic pH. This preliminary research can conclude that a lower pH is recommended for shampoos.
author2 Ng Kee Woei
author_facet Ng Kee Woei
Wu, Alicia Rosabee Yu Ling
format Final Year Project
author Wu, Alicia Rosabee Yu Ling
author_sort Wu, Alicia Rosabee Yu Ling
title Proteomics of hair: effects of pH on the hair shaft
title_short Proteomics of hair: effects of pH on the hair shaft
title_full Proteomics of hair: effects of pH on the hair shaft
title_fullStr Proteomics of hair: effects of pH on the hair shaft
title_full_unstemmed Proteomics of hair: effects of pH on the hair shaft
title_sort proteomics of hair: effects of ph on the hair shaft
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/165760
_version_ 1764208168923037696