Type I collagen gene knockdown by RNA interference.

Ever since synovium-derived mesenchymal stem cells (SMSCs) were first isolated from human synovial membrane tissues in 2001, they have been widely investigated for potential use in musculoskeletal therapy. SMSCs have superiority in chondrogenesis, which makes them good source of cell for cartilage r...

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Bibliographic Details
Main Author:	Li, Meng.
Other Authors:	Wang Dongan
Format:	Final Year Project
Language:	English
Published:	2009
Subjects:	DRNTU::Engineering::Chemical engineering::Biotechnology
Online Access:	http://hdl.handle.net/10356/16648
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Institution:	Nanyang Technological University
Language:	English

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record_format	dspace
spelling	sg-ntu-dr.10356-166482023-03-03T15:33:57Z Type I collagen gene knockdown by RNA interference. Li, Meng. Wang Dongan School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Ever since synovium-derived mesenchymal stem cells (SMSCs) were first isolated from human synovial membrane tissues in 2001, they have been widely investigated for potential use in musculoskeletal therapy. SMSCs have superiority in chondrogenesis, which makes them good source of cell for cartilage regeneration. It is crucial to inhibit the Collagen I expression of SMSC because Collagen I brings serious problems to cartilage regeneration. In this project, we try to silence the Collagen I expression of SMSCs by antisense technology. Recombinant adenovirus encoding shRNA which targets Collagen I were constructed and Collagen I knock down has been achieved in testing cells such as fibroblasts and osteoblasts. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T08:41:31Z 2009-05-27T08:41:31Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16648 en Nanyang Technological University 49 p. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle	DRNTU::Engineering::Chemical engineering::Biotechnology Li, Meng. Type I collagen gene knockdown by RNA interference.
description	Ever since synovium-derived mesenchymal stem cells (SMSCs) were first isolated from human synovial membrane tissues in 2001, they have been widely investigated for potential use in musculoskeletal therapy. SMSCs have superiority in chondrogenesis, which makes them good source of cell for cartilage regeneration. It is crucial to inhibit the Collagen I expression of SMSC because Collagen I brings serious problems to cartilage regeneration. In this project, we try to silence the Collagen I expression of SMSCs by antisense technology. Recombinant adenovirus encoding shRNA which targets Collagen I were constructed and Collagen I knock down has been achieved in testing cells such as fibroblasts and osteoblasts.
author2	Wang Dongan
author_facet	Wang Dongan Li, Meng.
format	Final Year Project
author	Li, Meng.
author_sort	Li, Meng.
title	Type I collagen gene knockdown by RNA interference.
title_short	Type I collagen gene knockdown by RNA interference.
title_full	Type I collagen gene knockdown by RNA interference.
title_fullStr	Type I collagen gene knockdown by RNA interference.
title_full_unstemmed	Type I collagen gene knockdown by RNA interference.
title_sort	type i collagen gene knockdown by rna interference.
publishDate	2009
url	http://hdl.handle.net/10356/16648
_version_	1759854205667901440

Type I collagen gene knockdown by RNA interference.

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