Shellfish pan-allergen tropomyosin: an investigation into its actin binding behaviour
Shellfish are commonly consumed worldwide, particularly in the Asia-Pacific region, due to their palatability and nutritional value. However, shellfish allergy which is a Type I (IgE- mediated) hypersensitivity affects 0.5 to 2.5% of the world population and can cause life- threatening anaphylaxis....
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| Format: | Final Year Project |
| Language: | English |
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Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/166938 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Shellfish are commonly consumed worldwide, particularly in the Asia-Pacific region, due to their palatability and nutritional value. However, shellfish allergy which is a Type I (IgE- mediated) hypersensitivity affects 0.5 to 2.5% of the world population and can cause life- threatening anaphylaxis. The major shellfish allergen is the heat-stable, coiled-coil protein tropomyosin (Tpm). As an actin-binding protein, the biochemistry of allergenic tropomyosin has been largely unexplored. In this study, we aim to gain some insights into the allergenic tropomyosin’s actin-binding behaviour. We successfully expressed and purified tropomyosin from two commonly consumed shellfish species, shrimp Lit v 1 and squid Tod p 1, as well as human Tpm 1.6 as a reference tropomyosin and analysed their actin-binding affinity. These two shellfish tropomyosins appear to have higher actin binding affinity than human tropomyosin, which sheds light on the fundamental biochemical difference between allergenic and non-allergenic tropomyosin. |
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