Alginate encapsulation improves probiotics survival in carbonated sodas and beers

Probiotic functionalization of non-dairy beverages has been garnering interest to provide dairy-sensitive populations with greater probiotic product varieties. The addition of probiotics into popularly consumed beverages-carbonated sodas and beers, presents an interesting challenge as the presence o...

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Main Authors: Tan, Li Ling, Ang, Kai Lin, Loo, Joachim Say Chye
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/168614
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1686142023-06-15T15:30:29Z Alginate encapsulation improves probiotics survival in carbonated sodas and beers Tan, Li Ling Ang, Kai Lin Loo, Joachim Say Chye School of Materials Science and Engineering Lee Kong Chian School of Medicine (LKCMedicine) Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Science::Medicine Alginate Probiotics Soda Beer Encapsulation Probiotic functionalization of non-dairy beverages has been garnering interest to provide dairy-sensitive populations with greater probiotic product varieties. The addition of probiotics into popularly consumed beverages-carbonated sodas and beers, presents an interesting challenge as the presence of acidic pH, hops-derived compounds, and ethanol have highly deleterious effects. Herein, alginate encapsulation was proposed to improve probiotics viability within sodas and beers. Three probiotics, namely Lacticaseibacillus rhamnosus GG, Escherichia coli Nissle 1917, and Bifidobacterium longum were encapsulated in alginate spheres and exposed to Coca-Cola, 7-Up, Tiger Beer, and Guinness under refrigerated, room temperature and simulated gastric fluid conditions. Results demonstrate that alginate encapsulation significantly improved the viabilities of all three probiotics in various beverages and conditions. Refrigerated storage better preserved probiotic viabilities and reduced the formation of the probiotic metabolic by-product, L-lactate, than at room temperature storage. Findings here could provide beverage manufacturers with a novel way to develop probiotic-sodas and probiotic-beers through encapsulation. Ministry of Education (MOE) Singapore Food Agency Published version SCJ Loo received funding from the Singapore Centre of Environmental Life Sciences Engineering (SCELSE) MOE/RCE: M4330019.C70 (www.scelse.sg), Ministry of Education AcRF-Tier 1 grant RG19/18 and RT08/19 (www.moe.gov.sg), the Singapore National Biofilm Consortium SNBC/ 2021/SF2/P04 (www.snbc.sg), and the Singapore Food Agency SFS_RND_SUFP_001_06 (www.sfa.gov.sg). 2023-06-13T06:42:28Z 2023-06-13T06:42:28Z 2023 Journal Article Tan, L. L., Ang, K. L. & Loo, J. S. C. (2023). Alginate encapsulation improves probiotics survival in carbonated sodas and beers. PLOS ONE, 18(3), e0283745-. https://dx.doi.org/10.1371/journal.pone.0283745 1932-6203 https://hdl.handle.net/10356/168614 10.1371/journal.pone.0283745 37000797 2-s2.0-85151329309 3 18 e0283745 en M4330019.C70 RG19/18 RT08/19 SNBC/2021/SF2/P04 SFS_RND_SUFP_001_06 PLOS ONE 10.21979/N9/LATSB5 © 2023 Tan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Alginate
Probiotics
Soda
Beer
Encapsulation
spellingShingle Science::Medicine
Alginate
Probiotics
Soda
Beer
Encapsulation
Tan, Li Ling
Ang, Kai Lin
Loo, Joachim Say Chye
Alginate encapsulation improves probiotics survival in carbonated sodas and beers
description Probiotic functionalization of non-dairy beverages has been garnering interest to provide dairy-sensitive populations with greater probiotic product varieties. The addition of probiotics into popularly consumed beverages-carbonated sodas and beers, presents an interesting challenge as the presence of acidic pH, hops-derived compounds, and ethanol have highly deleterious effects. Herein, alginate encapsulation was proposed to improve probiotics viability within sodas and beers. Three probiotics, namely Lacticaseibacillus rhamnosus GG, Escherichia coli Nissle 1917, and Bifidobacterium longum were encapsulated in alginate spheres and exposed to Coca-Cola, 7-Up, Tiger Beer, and Guinness under refrigerated, room temperature and simulated gastric fluid conditions. Results demonstrate that alginate encapsulation significantly improved the viabilities of all three probiotics in various beverages and conditions. Refrigerated storage better preserved probiotic viabilities and reduced the formation of the probiotic metabolic by-product, L-lactate, than at room temperature storage. Findings here could provide beverage manufacturers with a novel way to develop probiotic-sodas and probiotic-beers through encapsulation.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Tan, Li Ling
Ang, Kai Lin
Loo, Joachim Say Chye
format Article
author Tan, Li Ling
Ang, Kai Lin
Loo, Joachim Say Chye
author_sort Tan, Li Ling
title Alginate encapsulation improves probiotics survival in carbonated sodas and beers
title_short Alginate encapsulation improves probiotics survival in carbonated sodas and beers
title_full Alginate encapsulation improves probiotics survival in carbonated sodas and beers
title_fullStr Alginate encapsulation improves probiotics survival in carbonated sodas and beers
title_full_unstemmed Alginate encapsulation improves probiotics survival in carbonated sodas and beers
title_sort alginate encapsulation improves probiotics survival in carbonated sodas and beers
publishDate 2023
url https://hdl.handle.net/10356/168614
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