Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.

Helicobacter pylori, which causes gastric ulcers and cancers, contains the polymorphic virulence genes cagA and vacA. Differences in the cagA EPIYA motifs and polymorphisms of vacA signal (s), middle (m) and intermediate (i) regions were thought to be important in determining disease severity. This...

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Main Author: Goh, Hui Lin.
Other Authors: School of Biological Sciences
Format: Final Year Project
Language:English
Published: 2009
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Online Access:http://hdl.handle.net/10356/16898
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-168982023-02-28T18:02:28Z Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes. Goh, Hui Lin. School of Biological Sciences Singapore General Hospital Dr. Ling Khoon Lin DRNTU::Science::Biological sciences::Genetics Helicobacter pylori, which causes gastric ulcers and cancers, contains the polymorphic virulence genes cagA and vacA. Differences in the cagA EPIYA motifs and polymorphisms of vacA signal (s), middle (m) and intermediate (i) regions were thought to be important in determining disease severity. This study aims to determine cagA and vacA polymorphisms of H. pylori isolated from Singapore’s multi-ethnic population. Furthermore, a 12-year trend of antibiotic resistance from the isolates was studied. A total of 86 H. pylori isolates were obtained from patients with dyspeptic symptoms. Antibiotic resistance was determined by Etest; DNA was extracted from the isolates and allelic variation of virulence genes were determined by PCR and sequencing. High metronidazole resistance and increasing clarithromycin and levofloxacin resistance were observed through the 12 years. Chinese patients predominantly carried vacA s1/m2/i1 strains as compared to non-Chinese who mainly carried s1/m1/i1 (p<0.001). Furthermore, the Chinese isolates mainly possessed CagA with EPIYA-ABD motif, whereas non-Chinese isolates predominantly carried the EPIYA-ABC motif (p<0.001). With increased antibiotic resistance, enforcement of alternative treatments is essential. Marked polymorphic differences of cagA and vacA genotypes were observed between ethnic groups. Though vacA was not correlated with disease, cagA EPIYA genotypic differences may identify high-risk patients. Bachelor of Science in Biological Sciences 2009-05-28T09:01:55Z 2009-05-28T09:01:55Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16898 en Nanyang Technological University 36 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Genetics
spellingShingle DRNTU::Science::Biological sciences::Genetics
Goh, Hui Lin.
Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
description Helicobacter pylori, which causes gastric ulcers and cancers, contains the polymorphic virulence genes cagA and vacA. Differences in the cagA EPIYA motifs and polymorphisms of vacA signal (s), middle (m) and intermediate (i) regions were thought to be important in determining disease severity. This study aims to determine cagA and vacA polymorphisms of H. pylori isolated from Singapore’s multi-ethnic population. Furthermore, a 12-year trend of antibiotic resistance from the isolates was studied. A total of 86 H. pylori isolates were obtained from patients with dyspeptic symptoms. Antibiotic resistance was determined by Etest; DNA was extracted from the isolates and allelic variation of virulence genes were determined by PCR and sequencing. High metronidazole resistance and increasing clarithromycin and levofloxacin resistance were observed through the 12 years. Chinese patients predominantly carried vacA s1/m2/i1 strains as compared to non-Chinese who mainly carried s1/m1/i1 (p<0.001). Furthermore, the Chinese isolates mainly possessed CagA with EPIYA-ABD motif, whereas non-Chinese isolates predominantly carried the EPIYA-ABC motif (p<0.001). With increased antibiotic resistance, enforcement of alternative treatments is essential. Marked polymorphic differences of cagA and vacA genotypes were observed between ethnic groups. Though vacA was not correlated with disease, cagA EPIYA genotypic differences may identify high-risk patients.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Goh, Hui Lin.
format Final Year Project
author Goh, Hui Lin.
author_sort Goh, Hui Lin.
title Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
title_short Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
title_full Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
title_fullStr Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
title_full_unstemmed Analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
title_sort analysis of helicobacter pylori antimicrobial susceptibility and virulence genes.
publishDate 2009
url http://hdl.handle.net/10356/16898
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